Immunophenotyping in pemphigus reveals a T
IL-17
Pemphigus vulgaris
T-cell–B-cell interaction
autoantibodies
autoimmunity
bullous disease
desmoglein
follicular T cells
helper T cells
pemphigus foliaceus
Journal
The Journal of allergy and clinical immunology
ISSN: 1097-6825
Titre abrégé: J Allergy Clin Immunol
Pays: United States
ID NLM: 1275002
Informations de publication
Date de publication:
06 2021
06 2021
Historique:
received:
26
03
2020
revised:
19
10
2020
accepted:
13
11
2020
pubmed:
23
11
2020
medline:
5
10
2021
entrez:
22
11
2020
Statut:
ppublish
Résumé
T We aimed to characterize the exact phenotype of T cells responsible for the development of pemphigus. Whole transcriptome shotgun sequencing was performed to determine differential gene expression in pemphigus lesions and skin of healthy individuals. The cutaneous cytokine signature was further evaluated by real-time quantitative PCR. In peripheral blood, the distribution of T Transcriptome analysis of skin samples identified an IL-17A-dominated immune signature in patients with pemphigus, and Kyoto Encyclopedia of Genes and Genomes pathway analysis confirmed the dominance of the IL-17A signaling pathway. Increased expression of IL17A and associated cytokines was also detected by real-time quantitative PCR comparing lesional with perilesional or healthy skin. Interestingly, utilization of flow cytometry showed that patients with active pemphigus had elevated levels of circulating IL-17 Our findings show that T
Sections du résumé
BACKGROUND
T
OBJECTIVE
We aimed to characterize the exact phenotype of T cells responsible for the development of pemphigus.
METHODS
Whole transcriptome shotgun sequencing was performed to determine differential gene expression in pemphigus lesions and skin of healthy individuals. The cutaneous cytokine signature was further evaluated by real-time quantitative PCR. In peripheral blood, the distribution of T
RESULTS
Transcriptome analysis of skin samples identified an IL-17A-dominated immune signature in patients with pemphigus, and Kyoto Encyclopedia of Genes and Genomes pathway analysis confirmed the dominance of the IL-17A signaling pathway. Increased expression of IL17A and associated cytokines was also detected by real-time quantitative PCR comparing lesional with perilesional or healthy skin. Interestingly, utilization of flow cytometry showed that patients with active pemphigus had elevated levels of circulating IL-17
CONCLUSION
Our findings show that T
Identifiants
pubmed: 33221382
pii: S0091-6749(20)31624-9
doi: 10.1016/j.jaci.2020.11.008
pii:
doi:
Substances chimiques
Autoantibodies
0
Biomarkers
0
Desmoglein 1
0
Desmoglein 3
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2358-2369Informations de copyright
Copyright © 2020 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.