The antagonistic MYB paralogs RH1 and RH2 govern anthocyanin leaf markings in Medicago truncatula.
MYB regulator
Medicago truncatula
anthocyanin leaf marking
paralog antagonism
patterned pigmentation
Journal
The New phytologist
ISSN: 1469-8137
Titre abrégé: New Phytol
Pays: England
ID NLM: 9882884
Informations de publication
Date de publication:
03 2021
03 2021
Historique:
received:
20
10
2020
accepted:
14
11
2020
pubmed:
23
11
2020
medline:
15
5
2021
entrez:
22
11
2020
Statut:
ppublish
Résumé
Patterned leaf coloration in plants generates remarkable diversity in nature, but the underlying mechanisms remain largely unclear. Here, using Medicago truncatula leaf marking as a model, we show that the classic M. truncatula leaf anthocyanin spot trait depends on two R2R3 MYB paralogous regulators, RED HEART1 (RH1) and RH2. RH1 mainly functions as an anthocyanin biosynthesis activator that specifically determines leaf marking formation depending on its C-terminal activation motif. RH1 physically interacts with the M. truncatula bHLH protein MtTT8 and the WDR family member MtWD40-1, and this interaction facilitates RH1 function in leaf anthocyanin marking formation. RH2 has lost transcriptional activation activity, due to a divergent C-terminal domain, but retains the ability to interact with the same partners, MtTT8 and MtWD40-1, as RH1, thereby acting as a competitor in the regulatory complex and exerting opposite effects. Moreover, our results demonstrate that RH1 can activate its own expression and that RH2-mediated competition can repress RH1 expression. Our findings reveal the molecular mechanism of the antagonistic gene paralogs RH1 and RH2 in determining anthocyanin leaf markings in M. truncatula, providing a multidimensional paralogous-antagonistic regulatory paradigm for fine-tuning patterned pigmentation.
Identifiants
pubmed: 33222243
doi: 10.1111/nph.17097
pmc: PMC7986808
doi:
Substances chimiques
Anthocyanins
0
Plant Proteins
0
Transcription Factors
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
3330-3344Informations de copyright
© 2020 The Authors New Phytologist © 2020 New Phytologist Foundation.
Références
Plant J. 2019 Jun;98(6):1106-1119
pubmed: 30776165
Annu Rev Plant Biol. 2006;57:761-80
pubmed: 16669781
Nat Rev Genet. 2010 Feb;11(2):97-108
pubmed: 20051986
Plant Cell. 2014 Mar;26(3):962-80
pubmed: 24642943
Proc Natl Acad Sci U S A. 2010 Dec 21;107(51):22338-43
pubmed: 21135241
New Phytol. 2015 Jan;205(2):882-93
pubmed: 25329638
Plant J. 2010 Jan;61(2):259-70
pubmed: 19843313
Plant J. 2011 Mar;65(5):771-84
pubmed: 21235651
Bioinformatics. 2015 Jan 15;31(2):166-9
pubmed: 25260700
Plant Cell. 2019 Nov;31(11):2751-2767
pubmed: 31530734
Plant J. 2004 Aug;39(3):366-80
pubmed: 15255866
Curr Opin Plant Biol. 2001 Oct;4(5):447-56
pubmed: 11597504
Bioinformatics. 2009 Jul 15;25(14):1754-60
pubmed: 19451168
Plant Physiol. 2009 Nov;151(3):1114-29
pubmed: 19710231
Nature. 2011 Nov 16;480(7378):520-4
pubmed: 22089132
Plant J. 2009 Jul;59(1):136-49
pubmed: 19368693
J Theor Biol. 2001 Jun 7;210(3):385-8
pubmed: 11397139
Plant J. 2001 Nov;28(3):319-32
pubmed: 11722774
Trends Genet. 1997 Feb;13(2):67-73
pubmed: 9055608
Genetics. 2004 Mar;166(3):1463-502
pubmed: 15082563
Nat Plants. 2018 Dec;4(12):1017-1025
pubmed: 30397259
Cell. 2016 Apr 7;165(2):382-95
pubmed: 27040500
Proc Natl Acad Sci U S A. 2013 Jan 2;110(1):366-71
pubmed: 23248305
Plant J. 2008 Sep;55(6):940-53
pubmed: 18532978
Curr Biol. 2020 Mar 9;30(5):802-814.e8
pubmed: 32155414
Trends Plant Sci. 2005 Feb;10(2):63-70
pubmed: 15708343
Nat Rev Genet. 2007 Apr;8(4):272-85
pubmed: 17363976
Mol Plant. 2020 Jan 6;13(1):42-58
pubmed: 31678614
Bioinformatics. 2010 Jan 1;26(1):139-40
pubmed: 19910308
Nucleic Acids Res. 2011 May;39(10):e68
pubmed: 21398631
Plant Cell Rep. 2017 Feb;36(2):371-374
pubmed: 27834007
EMBO J. 1990 Feb;9(2):315-21
pubmed: 2303027
Plant Sci. 2011 Sep;181(3):219-29
pubmed: 21763532
Mol Plant. 2009 Jul;2(4):790-802
pubmed: 19825656
Proc Natl Acad Sci U S A. 2018 Jan 9;115(2):E334-E341
pubmed: 29279404
PLoS Comput Biol. 2018 Jan 26;14(1):e1005944
pubmed: 29373581
Plant Cell. 2016 Feb;28(2):304-13
pubmed: 26869697
Nat Methods. 2015 Apr;12(4):357-60
pubmed: 25751142
Science. 2016 May 20;352(6288):1009-13
pubmed: 27199432
Plant Cell. 2015 Oct;27(10):2860-79
pubmed: 26410301
Trends Plant Sci. 2015 Mar;20(3):176-85
pubmed: 25577424
New Phytol. 2016 May;210(3):905-21
pubmed: 26725247
Plant J. 2008 Apr;54(2):335-47
pubmed: 18208518
Nat Plants. 2018 Nov;4(11):930-941
pubmed: 30374094
Plant Physiol Biochem. 2013 Sep;70:123-32
pubmed: 23774374
Funct Plant Biol. 2012 Sep;39(8):619-638
pubmed: 32480814
EMBO J. 1987 Dec 1;6(12):3553-8
pubmed: 3428265
Trends Plant Sci. 2005 May;10(5):236-42
pubmed: 15882656