Optimizing the quality of clinical studies on oral microbiome: A practical guide for planning, performing, and reporting.
16S rDNA amplicon sequencing
clinical study design
dental plaque
metadata
microbiome
saliva
Journal
Periodontology 2000
ISSN: 1600-0757
Titre abrégé: Periodontol 2000
Pays: Denmark
ID NLM: 9313276
Informations de publication
Date de publication:
02 2021
02 2021
Historique:
pubmed:
24
11
2020
medline:
22
4
2021
entrez:
23
11
2020
Statut:
ppublish
Résumé
With this review, we aim to increase the quality standards for clinical studies with microbiome as an output parameter. We critically address the existing body of evidence for good quality practices in oral microbiome studies based on 16S rRNA gene amplicon sequencing. First, we discuss the usefulness of microbiome profile analyses. Is a microbiome study actually the best approach for answering the research question? This is followed by addressing the criteria for the most appropriate study design, sample size, and the necessary data (study metadata) that should be collected. Next, we evaluate the available evidence for best practices in sample collection, transport, storage, and DNA isolation. Finally, an overview of possible sequencing options (eg, 16S rRNA gene hypervariable regions, sequencing platforms), processing and data interpretation approaches, as well as requirements for meaningful data storage, sharing, and reporting are provided.
Identifiants
pubmed: 33226702
doi: 10.1111/prd.12359
pmc: PMC7756869
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Review
Langues
eng
Pagination
210-236Informations de copyright
© 2020 The Authors. Periodontology 2000 published by John Wiley & Sons Ltd.
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