Improvement of endothelial function by Gunnera tinctoria extract with antioxidant properties.
Antioxidant activity
Endothelial function
Gunnera tinctoria
Methanolic extract
Journal
Biological research
ISSN: 0717-6287
Titre abrégé: Biol Res
Pays: England
ID NLM: 9308271
Informations de publication
Date de publication:
23 Nov 2020
23 Nov 2020
Historique:
received:
19
05
2020
accepted:
18
11
2020
entrez:
24
11
2020
pubmed:
25
11
2020
medline:
20
1
2021
Statut:
epublish
Résumé
Gunnera tinctoria has been collected by Mapuche-Pewenche people for food and medicinal purposes. The high polyphenol content of methanolic extract from G. tinctoria leaves with chemical constituents such as ellagic acid and quercetin derivatives suggests its application to prevent endothelial dysfunction and oxidative stress. The aim of this study was to provide evidence of the protective effect of this extract on endothelial function by reducing oxidative stress induced by high D-glucose and H A methanolic extract with a high content of polyphenols (520 ± 30 mg gallic acid equivalents/g dry extract) was obtained from G. tinctoria leaves. Its main constituent was ellagic acid. The results of Ferric reducing antioxidant power and 2,2-diphenyl-1-picrylhydrazyl radical scavenging assays of the extract confirmed its antioxidant activity by inhibition pathway of radical species. The incubation of HUVECs with the extract decreased the apoptosis and reactive oxygen species (ROS) synthesis induced by high extracellular concentration of D-glucose or hydrogen peroxide. The extract increased endothelial NO levels and reduced vasoconstriction in human placental vessels. This study provides evidence about the antioxidant and endothelial protective properties of methanolic G. tinctoria leaf extract. The extract improves the availability of NO in HUVECs, inhibiting the production of ROS and vasoconstriction.
Sections du résumé
BACKGROUND
BACKGROUND
Gunnera tinctoria has been collected by Mapuche-Pewenche people for food and medicinal purposes. The high polyphenol content of methanolic extract from G. tinctoria leaves with chemical constituents such as ellagic acid and quercetin derivatives suggests its application to prevent endothelial dysfunction and oxidative stress. The aim of this study was to provide evidence of the protective effect of this extract on endothelial function by reducing oxidative stress induced by high D-glucose and H
RESULTS
RESULTS
A methanolic extract with a high content of polyphenols (520 ± 30 mg gallic acid equivalents/g dry extract) was obtained from G. tinctoria leaves. Its main constituent was ellagic acid. The results of Ferric reducing antioxidant power and 2,2-diphenyl-1-picrylhydrazyl radical scavenging assays of the extract confirmed its antioxidant activity by inhibition pathway of radical species. The incubation of HUVECs with the extract decreased the apoptosis and reactive oxygen species (ROS) synthesis induced by high extracellular concentration of D-glucose or hydrogen peroxide. The extract increased endothelial NO levels and reduced vasoconstriction in human placental vessels.
CONCLUSIONS
CONCLUSIONS
This study provides evidence about the antioxidant and endothelial protective properties of methanolic G. tinctoria leaf extract. The extract improves the availability of NO in HUVECs, inhibiting the production of ROS and vasoconstriction.
Identifiants
pubmed: 33228801
doi: 10.1186/s40659-020-00322-2
pii: 10.1186/s40659-020-00322-2
pmc: PMC7684749
doi:
Substances chimiques
Antioxidants
0
Plant Extracts
0
Polyphenols
0
Reactive Oxygen Species
0
Hydrogen Peroxide
BBX060AN9V
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
55Subventions
Organisme : Fondo Nacional de Desarrollo Científico y Tecnológico
ID : 1191651
Organisme : Corporación de Fomento de la Producción
ID : 13IDL223120
Organisme : gore BIO BIO
ID : R17A10003
Organisme : CONICYT PIA/APOYO CCTE
ID : AFB170007
Organisme : Fondequip
ID : EQM150025
Organisme : Fondequip
ID : AFB170007
Organisme : VRID-Asociativo
ID : 213.A84.014-1.0
Organisme : VRID-Asociativo
ID : 217.033.110-1.0
Organisme : VRID-Enlace
ID : 216.033.108-1.0
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