Implementation of High-Throughput Sequencing (HTS) in Aptamer Selection Technology.

Aptamers, Nucleotide / analysis Base Sequence Benchmarking Gene Library High-Throughput Nucleotide Sequencing / instrumentation Humans Ligands Nucleic Acid Conformation Nucleic Acids / chemistry Precision Medicine / methods SELEX Aptamer Technique / instrumentation
SELEX aptamer evolution high-throughput sequencing next-generation sequencing random library

Journal

International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791

Informations de publication

Date de publication:
20 Nov 2020
Historique:
received: 24 10 2020
revised: 18 11 2020
accepted: 19 11 2020
entrez: 25 11 2020
pubmed: 26 11 2020
medline: 9 3 2021
Statut: epublish

Résumé

Aptamers are nucleic acid ligands that bind specifically to a target of interest. Aptamers have gained in popularity due to their high potential for different applications in analysis, diagnostics, and therapeutics. The procedure called systematic evolution of ligands by exponential enrichment (SELEX) is used for aptamer isolation from large nucleic acid combinatorial libraries. The huge number of unique sequences implemented in the in vitro evolution in the SELEX process imposes the necessity of performing extensive sequencing of the selected nucleic acid pools. High-throughput sequencing (HTS) meets this demand of SELEX. Analysis of the data obtained from sequencing of the libraries produced during and after aptamer isolation provides an informative basis for precise aptamer identification and for examining the structure and function of nucleic acid ligands. This review discusses the technical aspects and the potential of the integration of HTS with SELEX.

Identifiants

DOI: 10.3390/ijms21228774 PMID: 33233573 PMC: PMC7699794
pubmed: 33233573
pii: ijms21228774
doi: 10.3390/ijms21228774
pmc: PMC7699794
pii:
doi:

Substances chimiques

Aptamers, Nucleotide 0
Ligands 0
Nucleic Acids 0

Types de publication

Journal Article Review

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Russian Foundation for Basic Research (RFBR)
ID : 18-34-20020

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Auteurs

Natalia Komarova (N)

Scientific-Manufacturing Complex Technological Centre, 1-7 Shokin Square, Zelenograd, 124498 Moscow, Russia.
ORCID: 0000-0002-6148-0971

Daria Barkova (D)

Scientific-Manufacturing Complex Technological Centre, 1-7 Shokin Square, Zelenograd, 124498 Moscow, Russia.

Alexander Kuznetsov (A)

Scientific-Manufacturing Complex Technological Centre, 1-7 Shokin Square, Zelenograd, 124498 Moscow, Russia.

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Classifications MeSH

questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléotides › Polynucléotides › Oligonucléotides › Aptamères nucléotidiques › Implementation of High-Throughput Sequencing...
questionsmedicales.fr › Sciences de l'information › Sources d'information › Services d'information › Documentation › Données de séquences moléculaires › Séquence nucléotidique › Implementation of High-Throughput Sequencing...
questionsmedicales.fr › Qualité, accès, évaluation des soins de santé › Qualité des soins de santé › Mécanismes d'évaluation des soins de santé › Évaluation de programme › Référenciation › Implementation of High-Throughput Sequencing...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Banque de gènes › Implementation of High-Throughput Sequencing...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Analyse de séquence › Séquençage nucléotidique à haut débit › Implementation of High-Throughput Sequencing...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Implementation of High-Throughput Sequencing...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Ligands › Implementation of High-Throughput Sequencing...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Conformation d'acide nucléique › Implementation of High-Throughput Sequencing...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › Implementation of High-Throughput Sequencing...
questionsmedicales.fr › Professions de santé › Médecine › Médecine clinique › Médecine de précision › Implementation of High-Throughput Sequencing...
questionsmedicales.fr › Technologie, industrie et agriculture › Technologie › Technologie pharmaceutique › Techniques de chimie synthétique › Techniques de chimie combinatoire › Technique SELEX › Implementation of High-Throughput Sequencing...