Comparison of Different Culture Methods for the Detection of Bacillus cereus Group in Cosmetics.

Agar Bacillus Bacillus cereus Colony Count, Microbial Cosmetics Food Microbiology

Journal

Journal of AOAC International
ISSN: 1944-7922
Titre abrégé: J AOAC Int
Pays: England
ID NLM: 9215446

Informations de publication

Date de publication:
01 Jul 2020
Historique:
received: 22 11 2019
revised: 29 01 2020
accepted: 31 01 2020
entrez: 26 11 2020
pubmed: 27 11 2020
medline: 29 6 2021
Statut: ppublish

Résumé

The U.S. Food and Drug Administration (FDA) Bacteriological Analytical Manual (BAM) reference culture method uses Modified Letheen Broth (MLB) for microbiological analyses for all types of cosmetic products. This study evaluated the effectiveness of MLB and Tryptone Azolectin Tween (TAT) broths using BAM reference culture method for cosmetics. Pure spore suspensions of B. cereus group members were experimentally spiked (McF: 0.5) into cosmetic products. After an aging period of 72 h, the products were analyzed using MLB and TAT broth. The enumeration of the cells was performed on B. cereus group selective plates Bacillus cereus rapid agar (BACARA) and Mannitol Yolk Polymyxin (MYP) plates. No statistical difference (p > 0.05) was found for the recovery of cells from the liquid products using either medium (MLB or TAT broth) and the selective plates. In solid/powder products, a combination of Tween 80 and MLB detected significantly more cells (p < 0.05) than combination of Tween 80 and TAT broth. The microbial counts on BACARA showed no significant differences (p > 0.05). However, when assessing cream/oil-based products, the number of cells detected by use of Tween 80/TAT broth was significantly higher than Tween 80/MLB, and MYP showed significantly higher counts than BACARA. This study showed that relative effectiveness of MLB vs. TAT for recovering of B. cereus group cells varied depending on the variety of formulation, and combination of preservatives of the tested cosmetic products. The findings suggest additional studies are needed to explore recovery of other relevant microorganisms that may contaminate cream/oil-based cosmetics.

Sections du résumé

BACKGROUND BACKGROUND
The U.S. Food and Drug Administration (FDA) Bacteriological Analytical Manual (BAM) reference culture method uses Modified Letheen Broth (MLB) for microbiological analyses for all types of cosmetic products.
OBJECTIVE OBJECTIVE
This study evaluated the effectiveness of MLB and Tryptone Azolectin Tween (TAT) broths using BAM reference culture method for cosmetics.
METHODS METHODS
Pure spore suspensions of B. cereus group members were experimentally spiked (McF: 0.5) into cosmetic products. After an aging period of 72 h, the products were analyzed using MLB and TAT broth. The enumeration of the cells was performed on B. cereus group selective plates Bacillus cereus rapid agar (BACARA) and Mannitol Yolk Polymyxin (MYP) plates.
RESULTS RESULTS
No statistical difference (p > 0.05) was found for the recovery of cells from the liquid products using either medium (MLB or TAT broth) and the selective plates. In solid/powder products, a combination of Tween 80 and MLB detected significantly more cells (p < 0.05) than combination of Tween 80 and TAT broth. The microbial counts on BACARA showed no significant differences (p > 0.05). However, when assessing cream/oil-based products, the number of cells detected by use of Tween 80/TAT broth was significantly higher than Tween 80/MLB, and MYP showed significantly higher counts than BACARA.
CONCLUSIONS CONCLUSIONS
This study showed that relative effectiveness of MLB vs. TAT for recovering of B. cereus group cells varied depending on the variety of formulation, and combination of preservatives of the tested cosmetic products. The findings suggest additional studies are needed to explore recovery of other relevant microorganisms that may contaminate cream/oil-based cosmetics.

Identifiants

DOI: 10.1093/jaoacint/qsaa016 PMID: 33241354
pubmed: 33241354
pii: 5865468
doi: 10.1093/jaoacint/qsaa016
doi:

Substances chimiques

Cosmetics 0
Agar 9002-18-0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

1129-1139

Informations de copyright

AOAC INTERNATIONAL 2020. This work is written by US Government employees and is in the public domain in the US.

Auteurs

Nadine Yossa (N)

IHRC, Inc, 2 Ravinia Drive, Atlanta, GA 30346, USA.
U.S. Food and Drug Administration, Office of Cosmetics and Colors, College Park, MD 20740, USA.
U.S. Food and Drug Administration, Office of Regulatory Science College Park, MD 20740, USA.

Son T Hoang (ST)

U.S. Food and Drug Administration, Office of Regulatory Science College Park, MD 20740, USA.
Oak Ridge Institute for Science and Education, Oak Ridge, TN 37831, USA.

Travis Canida (T)

U.S. Food and Drug Administration, Office of Regulatory Science College Park, MD 20740, USA.
U.S. Food and Drug Administration, Office of Analytics and Outreach, College Park, MD 20740, USA.

Rebecca Bell (R)

U.S. Food and Drug Administration, Office of Regulatory Science College Park, MD 20740, USA.

Sandra Tallent (S)

U.S. Food and Drug Administration, Office of Regulatory Science College Park, MD 20740, USA.

Eric Brown (E)

U.S. Food and Drug Administration, Office of Regulatory Science College Park, MD 20740, USA.

Thomas Hammack (T)

U.S. Food and Drug Administration, Office of Regulatory Science College Park, MD 20740, USA.

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Classifications MeSH

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questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets sporulés à Gram positif › Bacillaceae › Bacillus › Comparison of Different Culture Methods for...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets sporulés à Gram positif › Bacillaceae › Bacillus › Bacillus cereus › Comparison of Different Culture Methods for...
questionsmedicales.fr › Techniques d'investigation › Techniques de laboratoire clinique › Techniques microbiologiques › Numération de colonies microbiennes › Comparison of Different Culture Methods for...
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