Ecotopic over-expression of PoCHS from Paeonia ostii altered the fatty acids composition and content in Arabidopsis thaliana.
Paeonia ostii
chalcone synthase (CHS)
fatty acids
flavonoids
α-Linolenic acid
Journal
Physiologia plantarum
ISSN: 1399-3054
Titre abrégé: Physiol Plant
Pays: Denmark
ID NLM: 1256322
Informations de publication
Date de publication:
May 2021
May 2021
Historique:
revised:
22
11
2020
received:
15
08
2020
accepted:
23
11
2020
pubmed:
29
11
2020
medline:
24
4
2021
entrez:
28
11
2020
Statut:
ppublish
Résumé
Chalcone synthase (CHS) is the key enzyme in the flavonoid biosynthetic pathway and has been studied in many plants, but the function of the CHS gene has not been well characterized in Paeonia ostii. In this study, we obtained a CHS homolog gene from P. ostii, which possessed the putative conserved amino acids of chalcone synthase by multiple alignment analysis and demonstrated the highest expression in developing seeds. In vitro assays of the recombinant PoCHS protein confirmed enzymatic activity using malonyl-CoA and 4-coumaroyl-CoA as substrates, and the optimal pH and reaction temperature were 7.5 and 40 °C, respectively. Furthermore, ectopic over-expression of PoCHS in Arabidopsis up-regulated the expression levels of genes involved in seed development (ABI), glycolysis (PKp2, PDH-E1a, and SUS2/3), and especially fatty acid biosynthesis (BCCP2, CAC2, CDS2, FatA, and FAD3). This resulted in an increased unsaturated fatty acid content, especially α-linolenic acid, in transgenic Arabidopsis seeds. In this study, we examined the functions of CHS homolog of P. ostii and demonstrated its new function in seed fatty acid biosynthesis.
Substances chimiques
Fatty Acids
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
64-76Subventions
Organisme : By the Strategic Priority Research Program of the Chinese Academy of Sciences
ID : XDA23080601
Organisme : China Postdoctoral Science Foundation
ID : 2019M660855
Organisme : the National Natural Science Foundation of China
ID : 31471909
Informations de copyright
© 2020 Scandinavian Plant Physiology Society.
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