Arabidopsis SnRK1 negatively regulates phenylpropanoid metabolism via Kelch domain-containing F-box proteins.
Arabidopsis
Kelch repeat domain-containing F-box protein
Sucrose Non-Fermenting Related Kinase 1
energy starvation
phenylalanine ammonia lyase
phenylpropanoids
Journal
The New phytologist
ISSN: 1469-8137
Titre abrégé: New Phytol
Pays: England
ID NLM: 9882884
Informations de publication
Date de publication:
03 2021
03 2021
Historique:
received:
04
08
2020
accepted:
19
11
2020
pubmed:
1
12
2020
medline:
15
5
2021
entrez:
30
11
2020
Statut:
ppublish
Résumé
Phenylpropanoid metabolism represents a substantial metabolic sink for photosynthetically fixed carbon. The evolutionarily conserved Sucrose Non-Fermenting Related Kinase 1 (SnRK1) is a major metabolic sensor that reprograms metabolism upon carbon deprivation. However, it is not clear if and how the SnRK1-mediated sugar signaling pathway controls phenylpropanoid metabolism. Here, we show that Arabidopsis SnRK1 negatively regulates phenylpropanoid biosynthesis via a group of Kelch domain-containing F-box (KFB) proteins that are responsible for the ubiquitination and degradation of phenylalanine ammonia lyase (PAL). Downregulation of AtSnRK1 significantly promoted the accumulation of soluble phenolics and lignin polymers and drastically increased PAL cellular accumulation but only slightly altered its transcription level. Co-expression of SnRK1α with PAL in Nicotiana benthamiana leaves resulted in the severe attenuation of the latter's protein level, but protein interaction assays suggested PAL is not a direct substrate of SnRK1. Furthermore, up or downregulation of AtSnRK1 positively affected KFB
Substances chimiques
Arabidopsis Proteins
0
F-Box Proteins
0
Sucrose
57-50-1
Protein Serine-Threonine Kinases
EC 2.7.11.1
SnRK1 protein, Arabidopsis
EC 2.7.11.1
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
3345-3359Informations de copyright
© 2020 The Authors New Phytologist © 2020 New Phytologist Foundation.
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