Image Morphometric Analysis of B Cells and Plasma Cells in Erythema Nodosum Leprosum With Clinicopathological Correlation.
Adolescent
Adult
Aged
Antigens, CD20
/ metabolism
B-Lymphocytes
/ metabolism
Blood Cell Count
Child
Child, Preschool
Eosinophils
/ pathology
Erythema Nodosum
/ blood
Female
Humans
Immunohistochemistry
Infant
Leprosy, Lepromatous
/ blood
Leprosy, Paucibacillary
/ immunology
Male
Middle Aged
Neutrophils
Plasma Cells
/ metabolism
Syndecan-1
/ metabolism
T-Lymphocytes, Cytotoxic
/ pathology
T-Lymphocytes, Helper-Inducer
/ pathology
T-Lymphocytes, Regulatory
/ pathology
Young Adult
Journal
The American Journal of dermatopathology
ISSN: 1533-0311
Titre abrégé: Am J Dermatopathol
Pays: United States
ID NLM: 7911005
Informations de publication
Date de publication:
01 Oct 2021
01 Oct 2021
Historique:
pubmed:
3
12
2020
medline:
29
1
2022
entrez:
2
12
2020
Statut:
ppublish
Résumé
Erythema nodosum leprosum (ENL) occurs as an immunological complication of multibacillary leprosy (MBL). The pathogenesis of ENL is long considered to be a T-cell-mediated process. The role of B cells and plasma cells in ENL is not well described in the literature. Therefore, we investigated the B-cell and plasma cell infiltrates in the skin biopsies of biopsy-proven cases of ENL by immunohistochemistry and image morphometry and compared the result with paucibacillary leprosy and MBL. Moreover, we sought a correlation of the B-cell and plasma cell infiltrates with different clinical, hematological, histopathological, and bacteriological parameters as well as the T-cell subsets in the skin biopsies. Our study highlighted a significant reduction in the number of B cells from paucibacillary leprosy to MBL to ENL, although there was no significant variation in the plasma cell infiltrate. The plasma cell infiltrate correlated with absolute neutrophilia in the blood and the presence of eosinophils in the ENL lesions. Both B cells and plasma cells positively correlated with CD4-positive T-helper cells and the CD8-positive cytotoxic T cells. Besides, the B cells also correlated positively with the CD3-positive pan T cells in the biopsy and negatively correlated with the T-regulatory:T-cell ratio. Our results suggested the role of B cells and plasma cells even at the tissue level in the pathobiogenesis of ENL.
Identifiants
pubmed: 33264135
pii: 00000372-202110000-00004
doi: 10.1097/DAD.0000000000001860
doi:
Substances chimiques
Antigens, CD20
0
SDC1 protein, human
0
Syndecan-1
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
700-706Informations de copyright
Copyright © 2020 Wolters Kluwer Health, Inc. All rights reserved.
Déclaration de conflit d'intérêts
The authors declare no conflicts of interest.
Références
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