Rad21 Haploinsufficiency Prevents ALT-Associated Phenotypes in Zebrafish Brain Tumors.
Animals
Brain Neoplasms
/ genetics
Cell Cycle Proteins
/ genetics
Cell Line, Tumor
Chromosomal Proteins, Non-Histone
/ genetics
DNA Breaks, Double-Stranded
DNA Repair
/ genetics
DNA-Binding Proteins
/ genetics
Haploinsufficiency
/ genetics
HeLa Cells
Humans
Phenotype
Telomere
/ genetics
Zebrafish
/ genetics
Cohesins
ALT
DNA damage
brain tumors
cohesin
telomeres
zebrafish
Journal
Genes
ISSN: 2073-4425
Titre abrégé: Genes (Basel)
Pays: Switzerland
ID NLM: 101551097
Informations de publication
Date de publication:
30 11 2020
30 11 2020
Historique:
received:
27
10
2020
revised:
26
11
2020
accepted:
27
11
2020
entrez:
3
12
2020
pubmed:
4
12
2020
medline:
27
7
2021
Statut:
epublish
Résumé
Cohesin is a protein complex consisting of four core subunits responsible for sister chromatid cohesion in mitosis and meiosis, and for 3D genome organization and gene expression through the establishment of long distance interactions regulating transcriptional activity in the interphase. Both roles are important for telomere integrity, but the role of cohesin in telomere maintenance mechanisms in highly replicating cancer cells in vivo is poorly studied. Here we used a zebrafish model of brain tumor, which uses alternative lengthening of telomeres (ALT) as primary telomere maintenance mechanism to test whether haploinsufficiency for Rad21, a member of the cohesin ring, affects ALT development. We found that a reduction in Rad21 levels prevents ALT-associated phenotypes in zebrafish brain tumors and triggers an increase in
Identifiants
pubmed: 33266037
pii: genes11121442
doi: 10.3390/genes11121442
pmc: PMC7760354
pii:
doi:
Substances chimiques
Cell Cycle Proteins
0
Chromosomal Proteins, Non-Histone
0
DNA-Binding Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
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