The chaperonin CCT controls T cell receptor-driven 3D configuration of centrioles.
Journal
Science advances
ISSN: 2375-2548
Titre abrégé: Sci Adv
Pays: United States
ID NLM: 101653440
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
received:
13
03
2020
accepted:
19
10
2020
entrez:
3
12
2020
pubmed:
4
12
2020
medline:
15
4
2022
Statut:
epublish
Résumé
T lymphocyte activation requires the formation of immune synapses (IS) with antigen-presenting cells. The dynamics of membrane receptors, signaling scaffolds, microfilaments, and microtubules at the IS determine the potency of T cell activation and subsequent immune response. Here, we show that the cytosolic chaperonin CCT (chaperonin-containing TCP1) controls the changes in reciprocal orientation of the centrioles and polarization of the tubulin dynamics induced by T cell receptor in T lymphocytes forming an IS. CCT also controls the mitochondrial ultrastructure and the metabolic status of T cells, regulating the de novo synthesis of tubulin as well as posttranslational modifications (poly-glutamylation, acetylation, Δ1 and Δ2) of αβ-tubulin heterodimers, fine-tuning tubulin dynamics. These changes ultimately determine the function and organization of the centrioles, as shown by three-dimensional reconstruction of resting and stimulated primary T cells using cryo-soft x-ray tomography. Through this mechanism, CCT governs T cell activation and polarity.
Identifiants
pubmed: 33268369
pii: 6/49/eabb7242
doi: 10.1126/sciadv.abb7242
pmc: PMC7821906
pii:
doi:
Substances chimiques
Receptors, Antigen, T-Cell
0
Tubulin
0
Chaperonin Containing TCP-1
EC 3.6.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : European Research Council
ID : 294340
Pays : International
Organisme : Medical Research Council
ID : MC_UP_1201/16
Pays : United Kingdom
Informations de copyright
Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).
Références
Immunol Rev. 2002 Nov;189:84-97
pubmed: 12445267
J Neurosci. 2003 Apr 1;23(7):2655-64
pubmed: 12684451
Trends Cell Biol. 2015 Mar;25(3):125-36
pubmed: 25468068
Cell Stress Chaperones. 2009 Jan;14(1):23-31
pubmed: 18595008
Sci Rep. 2019 May 10;9(1):7187
pubmed: 31076588
EMBO J. 2011 Apr 6;30(7):1238-50
pubmed: 21326213
Proteins. 2014 May;82(5):703-7
pubmed: 24420718
Methods Cell Biol. 1999;61:13-34
pubmed: 9891307
Science. 2017 Dec 15;358(6369):1448-1453
pubmed: 29146868
Structure. 2013 Apr 2;21(4):540-9
pubmed: 23478063
Nat Rev Immunol. 2011 Sep 09;11(10):672-84
pubmed: 21904389
Adv Immunol. 2008;97:1-64
pubmed: 18501768
Structure. 2012 May 9;20(5):814-25
pubmed: 22503819
Nat Commun. 2011;2:282
pubmed: 21505438
J Proteome Res. 2014 Mar 7;13(3):1234-47
pubmed: 24512137
J Cell Biol. 2008 Sep 8;182(5):951-62
pubmed: 18779373
Sci Rep. 2017 Apr 04;7:45808
pubmed: 28374769
Cell Cycle. 2008 Apr 1;7(7):940-9
pubmed: 18414063
EMBO J. 2000 Nov 15;19(22):5971-9
pubmed: 11080144
Nat Commun. 2016 Apr 19;7:11389
pubmed: 27091106
EMBO J. 2011 Aug 16;30(19):3895-912
pubmed: 21847095
Mol Cell Proteomics. 2011 Jan;10(1):M110.003335
pubmed: 20807836
J Cell Sci. 2015 May 1;128(9):1824-34
pubmed: 25908846
J Immunol. 2010 Jul 1;185(1):367-75
pubmed: 20530266
J Cell Biol. 2009 Jun 29;185(7):1159-66
pubmed: 19564401
FEBS Lett. 2015 Sep 14;589(19 Pt A):2522-32
pubmed: 26140986
Dev Genet. 1993;14(6):460-70
pubmed: 8111974
J Immunol. 1984 Nov;133(5):2762-6
pubmed: 6384372
J Mol Biol. 2010 Aug 20;401(3):532-43
pubmed: 20600117
Nat Rev Immunol. 2009 May;9(5):324-37
pubmed: 19390566
J Cell Biol. 1989 Dec;109(6 Pt 1):2869-78
pubmed: 2592409
Nature. 2003 Dec 4;426(6966):570-4
pubmed: 14654843
J Cell Biol. 2013 Nov 25;203(4):563-74
pubmed: 24385483
Nature. 2006 Sep 28;443(7110):462-5
pubmed: 17006514
EMBO J. 2012 Nov 5;31(21):4140-52
pubmed: 22922463
Biomed Opt Express. 2016 Nov 14;7(12):5092-5103
pubmed: 28018727
Cell. 2018 Sep 6;174(6):1507-1521.e16
pubmed: 30100183
Science. 2012 Jan 27;335(6067):422-6
pubmed: 22282802
Mol Cell Proteomics. 2016 May;15(5):1740-60
pubmed: 26893027
Cell. 2013 Feb 14;152(4):768-77
pubmed: 23415226
J Exp Med. 2013 Oct 21;210(11):2415-33
pubmed: 24101378
Proc Natl Acad Sci U S A. 2012 Feb 21;109(8):2884-9
pubmed: 22308438
Nat Struct Mol Biol. 2011 Jan;18(1):14-9
pubmed: 21151115
Nat Commun. 2019 Jun 28;10(1):2865
pubmed: 31253771
Cell Rep. 2012 Oct 25;2(4):866-77
pubmed: 23041314
Trends Cell Biol. 2014 Jan;24(1):61-72
pubmed: 24119664
J Cell Sci. 1994 Jun;107 ( Pt 6):1529-43
pubmed: 7962195
Blood. 2010 Dec 23;116(26):5919-29
pubmed: 20881207