Reconstitution of oxaloacetate metabolism in the tricarboxylic acid cycle in Synechocystis sp. PCC 6803: discovery of important factors that directly affect the conversion of oxaloacetate.
Synechocystis sp. PCC 6803
citrate synthase
cyanobacteria
l-malate dehydrogenase
phosphoenolpyruvate carboxylase
tricarboxylic acid cycle
Journal
The Plant journal : for cell and molecular biology
ISSN: 1365-313X
Titre abrégé: Plant J
Pays: England
ID NLM: 9207397
Informations de publication
Date de publication:
03 2021
03 2021
Historique:
revised:
01
12
2020
received:
18
09
2020
accepted:
02
12
2020
pubmed:
7
12
2020
medline:
7
8
2021
entrez:
6
12
2020
Statut:
ppublish
Résumé
The tricarboxylic acid (TCA) cycle is one of the most important metabolic pathways in nature. Oxygenic photoautotrophic bacteria, cyanobacteria, have an unusual TCA cycle. The TCA cycle in cyanobacteria contains two unique enzymes that are not part of the TCA cycle in other organisms. In recent years, sustainable metabolite production from carbon dioxide using cyanobacteria has been looked at as a means to reduce the environmental burden of this gas. Among cyanobacteria, the unicellular cyanobacterium Synechocystis sp. PCC 6803 (Synechocystis 6803) is an optimal host for sustainable metabolite production. Recently, metabolite production using the TCA cycle in Synechocystis 6803 has been carried out. Previous studies revealed that the branch point of the oxidative and reductive TCA cycles, oxaloacetate metabolism, plays a key role in metabolite production. However, the biochemical mechanisms regulating oxaloacetate metabolism in Synechocystis 6803 are poorly understood. Concentrations of oxaloacetate in Synechocystis 6803 are extremely low, such that in vivo analysis of oxaloacetate metabolism does not seem realistic. Therefore, using purified enzymes, we reconstituted oxaloacetate metabolism in Synechocystis 6803 in vitro to reveal the regulatory mechanisms involved. Reconstitution of oxaloacetate metabolism revealed that pH, Mg
Substances chimiques
Fumarates
0
Magnesium Chloride
02F3473H9O
Oxaloacetic Acid
2F399MM81J
Phosphoenolpyruvate
73-89-2
Malate Dehydrogenase
EC 1.1.1.37
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1449-1458Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2020 Society for Experimental Biology and John Wiley & Sons Ltd.
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