The plastid proteome of the nonphotosynthetic chlorophycean alga Polytomella parva.
Amyloplast
Chlamydomonas reinhardtii
Chlorophycean algae
Nonphotosynthetic algae
Plastid proteome
Polytomella parva
Journal
Microbiological research
ISSN: 1618-0623
Titre abrégé: Microbiol Res
Pays: Germany
ID NLM: 9437794
Informations de publication
Date de publication:
Feb 2021
Feb 2021
Historique:
received:
22
07
2020
revised:
23
10
2020
accepted:
13
11
2020
pubmed:
8
12
2020
medline:
17
7
2021
entrez:
7
12
2020
Statut:
ppublish
Résumé
The unicellular, free-living, nonphotosynthetic chlorophycean alga Polytomella parva, closely related to Chlamydomonas reinhardtii and Volvox carteri, contains colorless, starch-storing plastids. The P. parva plastids lack all light-dependent processes but maintain crucial metabolic pathways. The colorless alga also lacks a plastid genome, meaning no transcription or translation should occur inside the organelle. Here, using an algal fraction enriched in plastids as well as publicly available transcriptome data, we provide a morphological and proteomic characterization of the P. parva plastid, ultimately identifying several plastid proteins, both by mass spectrometry and bioinformatic analyses. Data are available via ProteomeXchange with identifier PXD022051. Altogether these results led us to propose a plastid proteome for P. parva, i.e., a set of proteins that participate in carbohydrate metabolism; in the synthesis and degradation of starch, amino acids and lipids; in the biosynthesis of terpenoids and tetrapyrroles; in solute transport and protein translocation; and in redox homeostasis. This is the first detailed plastid proteome from a unicellular, free-living colorless alga.
Identifiants
pubmed: 33285428
pii: S0944-5013(20)30517-6
doi: 10.1016/j.micres.2020.126649
pii:
doi:
Substances chimiques
Amino Acids
0
Proteome
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
126649Informations de copyright
Copyright © 2020. Published by Elsevier GmbH.