Pandemic Vibrio cholerae shuts down site-specific recombination to retain an interbacterial defence mechanism.
Bacterial Proteins
/ classification
Base Sequence
Cholera
/ epidemiology
Humans
Models, Genetic
Multigene Family
Pandemics
Phylogeny
Recombination, Genetic
Sequence Homology, Nucleic Acid
Type VI Secretion Systems
/ genetics
Vibrio cholerae
/ classification
Virulence
/ genetics
Virulence Factors
/ genetics
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
07 12 2020
07 12 2020
Historique:
received:
17
01
2020
accepted:
06
11
2020
entrez:
8
12
2020
pubmed:
9
12
2020
medline:
29
12
2020
Statut:
epublish
Résumé
Vibrio cholerae is an aquatic microbe that can be divided into three subtypes: harmless environmental strains, localised pathogenic strains, and pandemic strains causing global cholera outbreaks. Each type has a contact-dependent type VI secretion system (T6SS) that kills neighbouring competitors by translocating unique toxic effector proteins. Pandemic isolates possess identical effectors, indicating that T6SS effectors may affect pandemicity. Here, we show that one of the T6SS gene clusters (Aux3) exists in two states: a mobile, prophage-like element in a small subset of environmental strains, and a truncated Aux3 unique to and conserved in pandemic isolates. Environmental Aux3 can be readily excised from and integrated into the genome via site-specific recombination, whereas pandemic Aux3 recombination is reduced. Our data suggest that environmental Aux3 acquisition conferred increased competitive fitness to pre-pandemic V. cholerae, leading to grounding of the element in the chromosome and propagation throughout the pandemic clade.
Identifiants
pubmed: 33288753
doi: 10.1038/s41467-020-20012-7
pii: 10.1038/s41467-020-20012-7
pmc: PMC7721734
doi:
Substances chimiques
Bacterial Proteins
0
Type VI Secretion Systems
0
Virulence Factors
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6246Subventions
Organisme : NIAID NIH HHS
ID : R01 AI139103
Pays : United States
Commentaires et corrections
Type : CommentIn
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