The regulatory role of vasoactive intestinal peptide in lacrimal gland ductal fluid secretion: A new piece of the puzzle in tear production.
Animals
Calcium
/ metabolism
Calcium Signaling
Carbachol
/ pharmacology
Chelating Agents
/ pharmacology
Cystic Fibrosis Transmembrane Conductance Regulator
/ deficiency
Egtazic Acid
/ analogs & derivatives
Intracellular Space
/ metabolism
Lacrimal Apparatus
/ metabolism
Mice, Knockout
Receptors, Vasoactive Intestinal Peptide, Type II
/ metabolism
Receptors, Vasoactive Intestinal Polypeptide, Type I
/ metabolism
Tears
/ metabolism
Vasoactive Intestinal Peptide
/ metabolism
Journal
Molecular vision
ISSN: 1090-0535
Titre abrégé: Mol Vis
Pays: United States
ID NLM: 9605351
Informations de publication
Date de publication:
2020
2020
Historique:
received:
23
05
2020
accepted:
04
12
2020
entrez:
14
12
2020
pubmed:
15
12
2020
medline:
27
8
2021
Statut:
epublish
Résumé
Vasoactive intestinal peptide (VIP) is an important regulator of lacrimal gland (LG) function although the effect of VIP on ductal fluid secretion is unknown. Therefore, the aim of the present study was to investigate the role of VIP in the regulation of fluid secretion of isolated LG ducts and to analyze the underlying intracellular mechanisms. LGs from wild-type (WT) and cystic fibrosis transmembrane conductance regulator (CFTR) knockout (KO) mice were used. Immunofluorescence was applied to confirm the presence of VIP receptors termed VPAC1 and VPAC2 in LG duct cells. Ductal fluid secretion evoked by VIP (100 nM) was measured in isolated ducts using videomicroscopy. Intracellular Ca VIP stimulation resulted in a robust and continuous fluid secretory response in isolated duct segments originated from WT mice. In contrast, CFTR KO ducts exhibited only a weak pulse-like secretion. A small but statistically significant increase was detected in the intracellular Ca These results suggest the importance of VIP in the regulation of ductal fluid secretion and the determining role of the adenylyl cyclase-cAMP-CFTR route in this process.
Substances chimiques
Chelating Agents
0
Receptors, Vasoactive Intestinal Peptide, Type II
0
Receptors, Vasoactive Intestinal Polypeptide, Type I
0
Cystic Fibrosis Transmembrane Conductance Regulator
126880-72-6
1,2-bis(2-aminophenoxy)ethane N,N,N',N'-tetraacetic acid acetoxymethyl ester
139890-68-9
Vasoactive Intestinal Peptide
37221-79-7
Egtazic Acid
526U7A2651
Carbachol
8Y164V895Y
Calcium
SY7Q814VUP
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
780-788Informations de copyright
Copyright © 2020 Molecular Vision.
Références
Curr Eye Res. 1989 Jun;8(6):619-36
pubmed: 2545411
Invest Ophthalmol Vis Sci. 1997 Mar;38(3):610-9
pubmed: 9071214
Am J Med. 1988 Oct;85(4):552-4
pubmed: 2845780
Exp Physiol. 2009 Feb;94(2):175-9
pubmed: 18931049
J Physiol. 2004 Apr 15;556(Pt 2):415-28
pubmed: 14978209
Invest Ophthalmol Vis Sci. 2016 Jul 1;57(8):3828-35
pubmed: 27438543
Invest Ophthalmol Vis Sci. 2010 Jun;51(6):2960-7
pubmed: 20107177
Cold Spring Harb Perspect Med. 2012 Jun;2(6):a009563
pubmed: 22675668
Mol Vis. 2011;17:2847-55
pubmed: 22128232
Eur J Ophthalmol. 2001 Jan-Mar;11(1):9-14
pubmed: 11284496
Invest Ophthalmol Vis Sci. 2007 Aug;48(8):3746-55
pubmed: 17652747
Br J Pharmacol. 2004 Feb;141(4):698-708
pubmed: 14744818
Neuropharmacology. 2006 Nov;51(6):1086-98
pubmed: 16930633
Invest Ophthalmol Vis Sci. 2006 May;47(5):1876-85
pubmed: 16638994
Pflugers Arch. 1972;337(4):299-309
pubmed: 4674879
Invest Ophthalmol Vis Sci. 2014 Jun 12;55(7):4360-7
pubmed: 24925876
J Anat. 2006 Feb;208(2):219-29
pubmed: 16441566
Recept Channels. 2002;8(3-4):137-53
pubmed: 12529932
Invest Ophthalmol Vis Sci. 2018 Jan 1;59(1):54-62
pubmed: 29305607
Ophthalmology. 1989 Nov;96(11):1624-30
pubmed: 2616148
Peptides. 2007 Sep;28(9):1631-9
pubmed: 17574305
Prog Retin Eye Res. 2009 May;28(3):155-77
pubmed: 19376264
Physiol Rev. 2012 Jan;92(1):39-74
pubmed: 22298651
Scand J Clin Lab Invest Suppl. 1969;107:51-6
pubmed: 5375727
Regul Pept. 2002 Oct 15;108(2-3):165-73
pubmed: 12220741
Cold Spring Harb Perspect Med. 2013 Jan 01;3(1):a009498
pubmed: 23284076
Nat Genet. 1993 May;4(1):35-41
pubmed: 7685652
J Membr Biol. 2009 Oct;231(2-3):65-78
pubmed: 19865788
Pflugers Arch. 2014 Aug;466(8):1487-99
pubmed: 24240699
J Physiol. 1981 Dec;321:557-69
pubmed: 6461755
Curr Eye Res. 2012 Aug;37(8):671-7
pubmed: 22578307