Probing Transient DNA Conformation Changes with an Intercalative Fluorescent Excimer.
DNA nanostructures
DNA transient conformation change
excimers
label free
plasmid
Journal
Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
Titre abrégé: Angew Chem Int Ed Engl
Pays: Germany
ID NLM: 0370543
Informations de publication
Date de publication:
15 03 2021
15 03 2021
Historique:
received:
28
10
2020
revised:
02
12
2020
pubmed:
15
12
2020
medline:
15
7
2021
entrez:
14
12
2020
Statut:
ppublish
Résumé
Variation of DNA conformation is important in regulating gene expression and mediating drug-DNA interactions. However, directly probing transient DNA conformation changes is challenging owing to the dynamic nature of this process. We show a label-free fluorescence method to monitor transient DNA conformation changes in DNA structures with various lengths and shapes using a DNA intercalator, K21. K21 can form transient excimers on the surface of DNA; the ratiometric emission of monomer and excimer correlate to DNA transient conformation stability in numerous DNA structures, including i-motifs, G-quadruplex structures, and single nucleotide mutation at random position. We analyzed the conformation dynamics of a single plasmid before and after enzyme digestion with confocal fluorescence microscopy. This method provides a label-free fluorescence strategy to probe transient conformation changes of DNA structures and has potential in uncovering transient genomic processes in living cells.
Identifiants
pubmed: 33314629
doi: 10.1002/anie.202014466
doi:
Substances chimiques
Fluorescent Dyes
0
DNA
9007-49-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6624-6630Informations de copyright
© 2020 Wiley-VCH GmbH.
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Deposition Number 2014005 (for K21) contains the supplementary crystallographic data for this paper. These data are provided free of charge by the joint Cambridge Crystallographic Data Centre and Fachinformationszentrum Karlsruhe Access Structures service www.ccdc.cam.ac.uk/structures.