Development of novel lab-on-a-chip platform for high-throughput radioimmunoassay.
Lab-on-a-chip
Microfluidics
RIA
Radioimmunoassay
Radioisotopes
Journal
Applied radiation and isotopes : including data, instrumentation and methods for use in agriculture, industry and medicine
ISSN: 1872-9800
Titre abrégé: Appl Radiat Isot
Pays: England
ID NLM: 9306253
Informations de publication
Date de publication:
Feb 2021
Feb 2021
Historique:
received:
08
05
2020
revised:
27
10
2020
accepted:
16
11
2020
pubmed:
15
12
2020
medline:
28
10
2021
entrez:
14
12
2020
Statut:
ppublish
Résumé
Radioimmunoassay (RIA) is an extremely specific and a highly sensitive type of immunoassay, but the long incubation time and generation of radioactive wastes limit the use of RIA. To complement these disadvantages of RIA, we suggest an advanced type of RIA based on a lab-on-a-chip (LOC) platform: μ-RIA. We designed a microfluidic chip for RIA and optimized the procedures of μ-RIA analysis, including surface modification, immunoreaction time, and washing. Based on the optimized conditions, we conducted a radioimmunoassay on the μ-RIA platform using a commercial RIA kit. With the μ-RIA, 5 min are adequate for analysis. The amount of reagent consumption is significantly reduced compared with conventional RIA. The standard curve with R
Identifiants
pubmed: 33316629
pii: S0969-8043(20)30667-9
doi: 10.1016/j.apradiso.2020.109526
pii:
doi:
Substances chimiques
Dimethylpolysiloxanes
0
baysilon
63148-62-9
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
109526Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.