Oleate-induced aggregation of LC3 at the trans-Golgi network is linked to a protein trafficking blockade.
Journal
Cell death and differentiation
ISSN: 1476-5403
Titre abrégé: Cell Death Differ
Pays: England
ID NLM: 9437445
Informations de publication
Date de publication:
05 2021
05 2021
Historique:
received:
28
05
2020
accepted:
25
11
2020
revised:
20
11
2020
pubmed:
19
12
2020
medline:
24
2
2022
entrez:
18
12
2020
Statut:
ppublish
Résumé
Oleate, the most abundant endogenous and dietary cis-unsaturated fatty acid, has the atypical property to cause the redistribution of microtubule-associated proteins 1A/1B light chain 3B (referred to as LC3) to the trans-Golgi network (TGN), as shown here. A genome-wide screen identified multiple, mostly Golgi transport-related genes specifically involved in the oleate-induced relocation of LC3 to the Golgi apparatus. Follow-up analyses revealed that oleate also caused the retention of secreted proteins in the TGN, as determined in two assays in which the secretion of proteins was synchronized, (i) an assay involving a thermosensitive vesicular stomatitis virus G (VSVG) protein that is retained in the endoplasmic reticulum (ER) until the temperature is lowered, and (ii) an isothermic assay involving the reversible retention of the protein of interest in the ER lumen and that was used both in vitro and in vivo. A pharmacological screen searching for agents that induce LC3 aggregation at the Golgi apparatus led to the identification of "oleate mimetics" that share the capacity to block conventional protein secretion. In conclusion, oleate represents a class of molecules that act on the Golgi apparatus to cause the recruitment of LC3 and to stall protein secretion.
Identifiants
pubmed: 33335289
doi: 10.1038/s41418-020-00699-3
pii: 10.1038/s41418-020-00699-3
pmc: PMC8167183
doi:
Substances chimiques
Lactosylceramides
0
Oleic Acid
2UMI9U37CP
lactotriaosylceramide
73467-80-8
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1733-1752Commentaires et corrections
Type : CommentIn
Références
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