Bioactive clerodane diterpenes of giant goldenrod (Solidago gigantea Ait.) root extract.
Anti-Infective Agents
/ chemistry
Bacillus subtilis
/ drug effects
Bacteria
/ drug effects
Cholinesterases
/ metabolism
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Diterpenes, Clerodane
/ isolation & purification
Enzyme Activation
/ drug effects
Enzyme Inhibitors
/ chemistry
Fusarium
/ drug effects
Plant Extracts
/ chemistry
Solidago
/ chemistry
Spectrometry, Mass, Electrospray Ionization
Xanthomonas
/ drug effects
Antibacterials and antifungals
Clerodane diterpenes
Fusarium avenaceum
Giant goldenrod (Solidago gigantea Ait.)
High-performance thin-layer chromatography – effect directed analysis
High-performance thin-layer chromatography – high-resolution mass spectrometry
Journal
Journal of chromatography. A
ISSN: 1873-3778
Titre abrégé: J Chromatogr A
Pays: Netherlands
ID NLM: 9318488
Informations de publication
Date de publication:
04 Jan 2021
04 Jan 2021
Historique:
received:
27
09
2020
revised:
13
11
2020
accepted:
16
11
2020
pubmed:
19
12
2020
medline:
31
12
2020
entrez:
18
12
2020
Statut:
ppublish
Résumé
Giant goldenrod (Solidago gigantea Ait.) root extract was screened for bioactive compounds by high-performance thin-layer chromatography (HPTLC), coupled with effect-directed analysis including antibacterial (Bacillus subtilis F1276, B. subtilis subsp. spizizenii, Aliivibrio fischeri and Xanthomonas euvesicatoria), antifungal (Fusarium avenaceum) and enzyme inhibition (acetyl- and butyrylcholinesterases, α- and β-glucosidases and α-amylase) assays. Compounds of six multipotent zones (Sg1-Sg6) were characterized by HPTLC-heated electrospray ionization-high-resolution mass spectrometry (HRMS) and HPTLC-Direct Analysis in Real Time-HRMS. Apart from zone Sg3, containing three compounds, a single characteristic compound was detectable in each bioactive zone. The bioassay-guided isolation using preparative-scale flash chromatography and high-performance liquid chromatography provided eight compounds that were identified by NMR spectroscopy as clerodane diterpenes. All isolates possessed inhibiting activity against at least one of the tested microorganisms.
Identifiants
pubmed: 33338903
pii: S0021-9673(20)31001-3
doi: 10.1016/j.chroma.2020.461727
pii:
doi:
Substances chimiques
Anti-Infective Agents
0
Diterpenes, Clerodane
0
Enzyme Inhibitors
0
Plant Extracts
0
Cholinesterases
EC 3.1.1.8
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
461727Informations de copyright
Copyright © 2020 The Author(s). Published by Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.