Redox activity of single bacteria revealed by electrochemical collision technique.
Electrochemical collision technique
Mediator
Redox activity
Single bacteria
Thionine
Ultramicroelectrode
Journal
Biosensors & bioelectronics
ISSN: 1873-4235
Titre abrégé: Biosens Bioelectron
Pays: England
ID NLM: 9001289
Informations de publication
Date de publication:
15 Mar 2021
15 Mar 2021
Historique:
received:
09
10
2020
revised:
12
12
2020
accepted:
16
12
2020
pubmed:
24
12
2020
medline:
15
5
2021
entrez:
23
12
2020
Statut:
ppublish
Résumé
This paper reports on an innovative strategy based on the electrochemical collision technique to quantify the redox activity of two bacterial species: the Gram-negative Escherichia coli and the Gram-positive Bacillus subtilis. Thionine (TH), as a redox mediator, was electrostatically adsorbed on bacterial surface and formed the bacterium-TH complexes. TH can receive electrons from bacterial metabolic pathways and be reduced. When a single bacterium-TH complex collides on the ultramicroelectrode, the reduced TH will be re-oxidized at certain potential and generate current spike. The frequency of the spikes is linearly proportional to the living bacteria concentration, and the redox activity of individual bacterium can be quantified by the charges enclosed in the current spike. The redox ability of Gram-negative E.coli to the TH mediator was 6.79 ± 0.26 × 10
Identifiants
pubmed: 33353760
pii: S0956-5663(20)30899-X
doi: 10.1016/j.bios.2020.112914
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
112914Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.