Specific Neuroligin3-αNeurexin1 signaling regulates GABAergic synaptic function in mouse hippocampus.
Animals
CA1 Region, Hippocampal
/ physiology
Calcium-Binding Proteins
/ physiology
Cell Adhesion Molecules, Neuronal
/ physiology
Female
GABAergic Neurons
/ physiology
Gene Knockdown Techniques
Hippocampus
/ physiology
Male
Membrane Proteins
/ physiology
Mice
Mice, Inbred C57BL
Nerve Tissue Proteins
/ physiology
Neural Cell Adhesion Molecules
/ physiology
Synapses
/ physiology
electrophysiology
hippocampus
inhibitory interneuron
mouse
neuroscience
trans-synaptic adhesion
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
23 12 2020
23 12 2020
Historique:
received:
01
06
2020
accepted:
08
12
2020
entrez:
23
12
2020
pubmed:
24
12
2020
medline:
23
3
2021
Statut:
epublish
Résumé
Synapse formation and regulation require signaling interactions between pre- and postsynaptic proteins, notably cell adhesion molecules (CAMs). It has been proposed that the functions of neuroligins (Nlgns), postsynaptic CAMs, rely on the formation of trans-synaptic complexes with neurexins (Nrxns), presynaptic CAMs. Nlgn3 is a unique Nlgn isoform that localizes at both excitatory and inhibitory synapses. However, Nlgn3 function mediated via Nrxn interactions is unknown. Here we demonstrate that Nlgn3 localizes at postsynaptic sites apposing vesicular glutamate transporter 3-expressing (VGT3+) inhibitory terminals and regulates VGT3+ inhibitory interneuron-mediated synaptic transmission in mouse organotypic slice cultures. Gene expression analysis of interneurons revealed that the αNrxn1+AS4 splice isoform is highly expressed in VGT3+ interneurons as compared with other interneurons. Most importantly, postsynaptic Nlgn3 requires presynaptic αNrxn1+AS4 expressed in VGT3+ interneurons to regulate inhibitory synaptic transmission. Our results indicate that specific Nlgn-Nrxn signaling generates distinct functional properties at synapses.
Identifiants
pubmed: 33355091
doi: 10.7554/eLife.59545
pii: 59545
pmc: PMC7758064
doi:
pii:
Substances chimiques
Calcium-Binding Proteins
0
Cell Adhesion Molecules, Neuronal
0
Membrane Proteins
0
Nerve Tissue Proteins
0
Neural Cell Adhesion Molecules
0
Nrxn1 protein, mouse
0
neuroligin 3
0
Banques de données
GEO
['GSE150989']
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Japan Society for the Promotion of Science
ID : 19100005
Pays : International
Organisme : NINDS NIH HHS
ID : R01 NS085215
Pays : United States
Organisme : NIMH NIH HHS
ID : F30 MH122146
Pays : United States
Organisme : Niigata University
ID : G2905
Pays : International
Organisme : NIGMS NIH HHS
ID : T32 GM107000
Pays : United States
Organisme : Japan Society for the Promotion of Science
ID : 15K06732
Pays : International
Organisme : Japan Society for the Promotion of Science
ID : 20H03349
Pays : International
Informations de copyright
© 2020, Uchigashima et al.
Déclaration de conflit d'intérêts
MU, KK, ED, AC, TW, DK, MA, TL, KS, TS, TU, YI, MW, KF No competing interests declared
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