Structural, biosynthetic and serological cross-reactive elucidation of capsular polysaccharides from Streptococcus pneumoniae serogroup 28.
Amino Acid Sequence
Bacterial Capsules
/ chemistry
Cross Reactions
Glycosyltransferases
/ chemistry
Immune Sera
/ immunology
Latex Fixation Tests
Magnetic Resonance Spectroscopy
Molecular Structure
Molecular Weight
Polysaccharides, Bacterial
/ biosynthesis
Serogroup
Streptococcus pneumoniae
/ chemistry
Biosynthesis
Capsular polysaccharides;Serogroup 28
Serological cross-reactivity
Streptococcus pneumoniae
Structural characterization
Journal
Carbohydrate polymers
ISSN: 1879-1344
Titre abrégé: Carbohydr Polym
Pays: England
ID NLM: 8307156
Informations de publication
Date de publication:
15 Feb 2021
15 Feb 2021
Historique:
received:
14
08
2020
revised:
21
10
2020
accepted:
23
10
2020
entrez:
28
12
2020
pubmed:
29
12
2020
medline:
15
4
2021
Statut:
ppublish
Résumé
Capsular polysaccharides (CPS) are the key virulent factors in the pathogenesis of Streptococcus pneumoniae. The previously unknown CPS structures of the pneumococcal serotype 28F and 28A were thoroughly characterized by NMR spectroscopy, chemical analysis and AF4-MALS-dRI. The following repeat unit structures were determined: -4)[α-l-Rhap-[4-P-2-Gro]]-(1-3)-α-d-Sug-[6-P-Cho]-(1-3)-β-l-Rhap-[2-OAc]-(1-4)-β-d-Glcp-(1-; 28F: Sug = Glcp, Mw: 540.5 kDa; 28A: Sug = GlcpNAc, Mw: 421.9 kDa; The correlation of CPS structures with biosynthesis showed that glycosyltransferase WciU in serotypes 28F and 28A had different sugar donor specificity toward α-d-Glcp and α-d-GlcNAcp, respectively. Furthermore, latex agglutination tests of de-OAc and de-PO
Identifiants
pubmed: 33357884
pii: S0144-8617(20)31496-X
doi: 10.1016/j.carbpol.2020.117323
pii:
doi:
Substances chimiques
Immune Sera
0
Polysaccharides, Bacterial
0
Glycosyltransferases
EC 2.4.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
117323Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.