Deciphering the pathogenesis of the COL4-related hematuric nephritis: A genotype/phenotype study.


Journal

Molecular genetics & genomic medicine
ISSN: 2324-9269
Titre abrégé: Mol Genet Genomic Med
Pays: United States
ID NLM: 101603758

Informations de publication

Date de publication:
02 2021
Historique:
revised: 18 11 2020
received: 14 04 2020
accepted: 24 11 2020
pubmed: 29 12 2020
medline: 5 8 2021
entrez: 28 12 2020
Statut: ppublish

Résumé

Alport syndrome (ATS) is a hereditary progressive hematuric nephropathy associated with sensorineural deafness and ocular abnormalities, which is caused by mutations in the COL4A5 gene (X-linked ATS) and in two autosomal genes, COL4A4 and COL4A3, responsible of both recessive ATS and, when present in heterozygosity, of a spectrum of phenotypes ranging from isolated hematuria to frank renal disease. Retrospective analysis of the clinical and genetic features of 76 patients from 34 unrelated ATS families (11 with mutations in COL4A5, 11 in COL4A3, and 12 in COL4A4) and genotype/phenotype correlation for the COL4A3/COL4A4 heterozygotes (34 patients from 14 families). Eight (24%) of the 34 heterozygous COL4A3 and COL4A4 carriers developed renal failure at a mean age of 57 years, with a significantly lower risk than hemizygous COL4A5 or double heterozygous COL4A3/COL4A4 carriers (p < 0.01), but not different from that of the heterozygous COL4A5 females (p = 0.6). Heterozygous carriers of frameshift/splicing variants in COL4A3/COL4A4 presented a higher risk of developing renal failure than those with missense variants in the glycine domains (p = 0.015). The renal functional prognosis of patients with COL4A3/COL4A4-positive ATS recapitulates that of the X-linked ATS forms, with differences between heterozygous vs. double heterozygous patients and between carriers of loss-of-function vs. missense variants.

Sections du résumé

BACKGROUND
Alport syndrome (ATS) is a hereditary progressive hematuric nephropathy associated with sensorineural deafness and ocular abnormalities, which is caused by mutations in the COL4A5 gene (X-linked ATS) and in two autosomal genes, COL4A4 and COL4A3, responsible of both recessive ATS and, when present in heterozygosity, of a spectrum of phenotypes ranging from isolated hematuria to frank renal disease.
METHODS
Retrospective analysis of the clinical and genetic features of 76 patients from 34 unrelated ATS families (11 with mutations in COL4A5, 11 in COL4A3, and 12 in COL4A4) and genotype/phenotype correlation for the COL4A3/COL4A4 heterozygotes (34 patients from 14 families).
RESULTS
Eight (24%) of the 34 heterozygous COL4A3 and COL4A4 carriers developed renal failure at a mean age of 57 years, with a significantly lower risk than hemizygous COL4A5 or double heterozygous COL4A3/COL4A4 carriers (p < 0.01), but not different from that of the heterozygous COL4A5 females (p = 0.6). Heterozygous carriers of frameshift/splicing variants in COL4A3/COL4A4 presented a higher risk of developing renal failure than those with missense variants in the glycine domains (p = 0.015).
CONCLUSION
The renal functional prognosis of patients with COL4A3/COL4A4-positive ATS recapitulates that of the X-linked ATS forms, with differences between heterozygous vs. double heterozygous patients and between carriers of loss-of-function vs. missense variants.

Identifiants

pubmed: 33369211
doi: 10.1002/mgg3.1576
pmc: PMC8077073
doi:

Substances chimiques

Collagen Type IV 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e1576

Informations de copyright

© 2020 The Authors. Molecular Genetics & Genomic Medicine published by Wiley Periodicals LLC.

Références

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Auteurs

Vera Uliana (V)

Medical Genetics, University Hospital of Parma, Parma, Italy.

Paola Sebastio (P)

Medical Genetics, University Hospital of Parma, Parma, Italy.

Matteo Riva (M)

Medical Genetics, Department of Medicine and Surgery, University of Parma, Parma, Italy.

Diana Carli (D)

Medical Genetics, University Hospital "Città della Salute", Torino, Italy.

Claudio Ruberto (C)

Pediatrics, University Hospital of Parma, Parma, Italy.

Laura Bianchi (L)

Pediatrics, University Hospital of Parma, Parma, Italy.

Claudio Graziano (C)

Medical Genetics, S. Orsola-Malpighi University Hospital, Bologna, Italy.

Irene Capelli (I)

Nephrology, S. Orsola-Malpighi University Hospital, Bologna, Italy.

Flavio Faletra (F)

Medical Genetics, I.R.C.C.S. Burlo Garofolo, Trieste, Italy.

Roberto Pillon (R)

Medical Genetics, University of Trieste, Trieste, Italy.

Teresa Mattina (T)

Medical Genetics, Centro di Riferimento Regionale per la Diagnosi e Cura della Malattie Genetiche, Catania, Italy.

Alberto Sensi (A)

Medical Genetics, Maurizio Bufalini Hospital, Cesena, Italy.

Francesco Bonatti (F)

Medical Genetics, Department of Medicine and Surgery, University of Parma, Parma, Italy.

Antonio Percesepe (A)

Medical Genetics, University Hospital of Parma, Parma, Italy.
Medical Genetics, Department of Medicine and Surgery, University of Parma, Parma, Italy.

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Classifications MeSH