Peripheral Blood Immune Profiling of Convalescent Plasma Donors Reveals Alterations in Specific Immune Subpopulations Even at 2 Months Post SARS-CoV-2 Infection.


Journal

Viruses
ISSN: 1999-4915
Titre abrégé: Viruses
Pays: Switzerland
ID NLM: 101509722

Informations de publication

Date de publication:
25 12 2020
Historique:
received: 22 11 2020
revised: 19 12 2020
accepted: 22 12 2020
entrez: 30 12 2020
pubmed: 31 12 2020
medline: 20 1 2021
Statut: epublish

Résumé

Immune profiling of patients with COVID-19 has shown that SARS-CoV-2 causes severe lymphocyte deficiencies (e.g., lymphopenia, decreased numbers, and exhaustion of T cells) and increased levels of pro-inflammatory monocytes. Peripheral blood (PB) samples from convalescent plasma (CP) donors, COVID-19 patients, and control subjects were analyzed by multiparametric flow cytometry, allowing the identification of a wide panel of immune cells, comprising lymphocytes (T, B, natural killer (NK) and NKT cells), monocytes, granulocytes, and their subsets. Compared to active COVID-19 patients, our results revealed that the immune profile of recovered donors was restored for most subpopulations. Nevertheless, even 2 months after recovery, CP donors still had reduced levels of CD4

Identifiants

pubmed: 33375675
pii: v13010026
doi: 10.3390/v13010026
pmc: PMC7824046
pii:
doi:

Substances chimiques

Antibodies, Viral 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

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Auteurs

Nikolaos Orologas-Stavrou (N)

Department of Biology, School of Sciences, National and Kapodistrian University of Athens, 15784 Athens, Greece.

Marianna Politou (M)

Hematology Laboratory-Blood Bank, Aretaieion Hospital, School of Medicine, National and Kapodistrian University of Athens, 11528 Athens, Greece.

Pantelis Rousakis (P)

Department of Biology, School of Sciences, National and Kapodistrian University of Athens, 15784 Athens, Greece.

Ioannis V Kostopoulos (IV)

Department of Biology, School of Sciences, National and Kapodistrian University of Athens, 15784 Athens, Greece.

Ioannis Ntanasis-Stathopoulos (I)

Department of Clinical Therapeutics, Alexandra General Hospital, School of Medicine, National and Kapodistrian University of Athens, 11528 Athens, Greece.

Edison Jahaj (E)

First Department of Critical Care Medicine and Pulmonary Services, Evangelismos General Hospital, National and Kapodistrian University of Athens, 11527 Athens, Greece.

Eleni Tsiligkeridou (E)

Department of Clinical Therapeutics, Alexandra General Hospital, School of Medicine, National and Kapodistrian University of Athens, 11528 Athens, Greece.

Maria Gavriatopoulou (M)

Department of Clinical Therapeutics, Alexandra General Hospital, School of Medicine, National and Kapodistrian University of Athens, 11528 Athens, Greece.

Efstathios Kastritis (E)

Department of Clinical Therapeutics, Alexandra General Hospital, School of Medicine, National and Kapodistrian University of Athens, 11528 Athens, Greece.

Anastasia Kotanidou (A)

First Department of Critical Care Medicine and Pulmonary Services, Evangelismos General Hospital, National and Kapodistrian University of Athens, 11527 Athens, Greece.

Meletios-Athanasios Dimopoulos (MA)

Department of Clinical Therapeutics, Alexandra General Hospital, School of Medicine, National and Kapodistrian University of Athens, 11528 Athens, Greece.

Ourania E Tsitsilonis (OE)

Department of Biology, School of Sciences, National and Kapodistrian University of Athens, 15784 Athens, Greece.

Evangelos Terpos (E)

Department of Clinical Therapeutics, Alexandra General Hospital, School of Medicine, National and Kapodistrian University of Athens, 11528 Athens, Greece.

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