Insights into the Interaction of LVV-Hemorphin-7 with Angiotensin II Type 1 Receptor.

Angiotensin II / metabolism HEK293 Cells Hemoglobins / metabolism Humans Molecular Docking Simulation Molecular Dynamics Simulation Peptide Fragments / metabolism Receptor, Angiotensin, Type 1 / metabolism
AT1R AngII LVV-hemorphin-7 NanoBRET PAM molecular docking molecular dynamics

Journal

International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791

Informations de publication

Date de publication:
28 Dec 2020
Historique:
received: 29 10 2020
revised: 17 12 2020
accepted: 24 12 2020
entrez: 31 12 2020
pubmed: 1 1 2021
medline: 8 9 2021
Statut: epublish

Résumé

Hemorphins are known for their role in the control of blood pressure. Recently, we revealed the positive modulation of the angiotensin II (AngII) type 1 receptor (AT1R) by LVV-hemorphin-7 (LVV-H7) in human embryonic kidney (HEK293) cells. Here, we examined the molecular binding behavior of LVV-H7 on AT1R and its effect on AngII binding using a nanoluciferase-based bioluminescence resonance energy transfer (NanoBRET) assay in HEK293FT cells, as well as molecular docking and molecular dynamics (MD) studies. Saturation and real-time kinetics supported the positive effect of LVV-H7 on the binding of AngII. While the competitive antagonist olmesartan competed with AngII binding, LVV-H7 slightly, but significantly, decreased AngII's k

Identifiants

DOI: 10.3390/ijms22010209 PMID: 33379211 PMC: PMC7795518
pubmed: 33379211
pii: ijms22010209
doi: 10.3390/ijms22010209
pmc: PMC7795518
pii:
doi:

Substances chimiques

Hemoglobins 0
Peptide Fragments 0
Receptor, Angiotensin, Type 1 0
Angiotensin II 11128-99-7
LVV-hemorphin-7 75808-66-1

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : United Arab Emirates University
ID : 31R235 and 31S243
Organisme : Australian Research Council
ID : LP160100857

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Auteurs

Amanat Ali (A)

Department of Biology, College of Science, United Arab Emirates University, Al Ain PO Box 15551, UAE.

Elizabeth K M Johnstone (EKM)

Molecular Endocrinology and Pharmacology, Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands, WA 6009, Australia.
Centre for Medical Research, The University of Western Australia, Crawley, WA 6009, Australia.
Australian Research Council Centre for Personalised Therapeutics Technologies, Canberra, NSW 2609, Australia.
ORCID: 0000-0001-7990-371X

Bincy Baby (B)

Department of Biology, College of Science, United Arab Emirates University, Al Ain PO Box 15551, UAE.

Heng B See (HB)

Molecular Endocrinology and Pharmacology, Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands, WA 6009, Australia.
Centre for Medical Research, The University of Western Australia, Crawley, WA 6009, Australia.
Australian Research Council Centre for Personalised Therapeutics Technologies, Canberra, NSW 2609, Australia.

Angela Song (A)

School of Biomedical Sciences, Faculty of Medicine, The University of Queensland, St Lucia, QLD 4072, Australia.

K Johan Rosengren (KJ)

School of Biomedical Sciences, Faculty of Medicine, The University of Queensland, St Lucia, QLD 4072, Australia.
ORCID: 0000-0002-5007-8434

Kevin D G Pfleger (KDG)

Molecular Endocrinology and Pharmacology, Harry Perkins Institute of Medical Research, QEII Medical Centre, Nedlands, WA 6009, Australia.
Centre for Medical Research, The University of Western Australia, Crawley, WA 6009, Australia.
Australian Research Council Centre for Personalised Therapeutics Technologies, Canberra, NSW 2609, Australia.
Dimerix Limited, Nedlands, WA 6009, Australia.
ORCID: 0000-0001-8261-9189

Mohammed Akli Ayoub (MA)

Department of Biology, College of Science, United Arab Emirates University, Al Ain PO Box 15551, UAE.
Zayed Center for Health Sciences, United Arab Emirates University, Al Ain PO Box 15551, UAE.
ORCID: 0000-0003-0022-578X

Ranjit Vijayan (R)

Department of Biology, College of Science, United Arab Emirates University, Al Ain PO Box 15551, UAE.
ORCID: 0000-0002-3830-7409

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Classifications MeSH

questionsmedicales.fr › Facteurs biologiques › Médiateurs de l'inflammation › Autacoïdes › Angiotensines › Angiotensine-II › Insights into the Interaction of...
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules HEK293 › Insights into the Interaction of...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Hémoprotéines › Globines › Hémoglobines › Insights into the Interaction of...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Insights into the Interaction of...
questionsmedicales.fr › Sciences de l'information › Méthodologies informatiques › Simulation numérique › Simulation de docking moléculaire › Insights into the Interaction of...
questionsmedicales.fr › Sciences de l'information › Méthodologies informatiques › Simulation numérique › Simulation de dynamique moléculaire › Insights into the Interaction of...
questionsmedicales.fr › Acides aminés, peptides et protéines › Peptides › Fragments peptidiques › Insights into the Interaction of...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Récepteurs de surface cellulaire › Récepteurs peptidiques › Récepteurs aux angiotensines › Récepteur de type 1 à l'angiotensine-II › Insights into the Interaction of...