Comprehensive analysis of TCR repertoire in COVID-19 using single cell sequencing.
COVID-19
TCR bias
TCR repertoire
scTCR-seq
Journal
Genomics
ISSN: 1089-8646
Titre abrégé: Genomics
Pays: United States
ID NLM: 8800135
Informations de publication
Date de publication:
03 2021
03 2021
Historique:
received:
02
10
2020
revised:
20
12
2020
accepted:
27
12
2020
pubmed:
1
1
2021
medline:
9
3
2021
entrez:
31
12
2020
Statut:
ppublish
Résumé
T-cell receptor (TCR) is crucial in T cell-mediated virus clearance. To date, TCR bias has been observed in various diseases. However, studies on the TCR repertoire of COVID-19 patients are lacking. Here, we used single-cell V(D)J sequencing to conduct comparative analyses of TCR repertoire between 12 COVID-19 patients and 6 healthy controls, as well as other virus-infected samples. We observed distinct T cell clonal expansion in COVID-19. Further analysis of VJ gene combination revealed 6 VJ pairs significantly increased, while 139 pairs significantly decreased in COVID-19 patients. When considering the VJ combination of α and β chains at the same time, the combination with the highest frequency on COVID-19 was TRAV12-2-J27-TRBV7-9-J2-3. Besides, preferential usage of V and J gene segments was also observed in samples infected by different viruses. Our study provides novel insights on TCR in COVID-19, which contribute to our understanding of the immune response induced by SARS-CoV-2.
Identifiants
pubmed: 33383142
pii: S0888-7543(20)32083-8
doi: 10.1016/j.ygeno.2020.12.036
pmc: PMC7833309
pii:
doi:
Substances chimiques
Receptors, Antigen, T-Cell
0
Types de publication
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
456-462Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
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