Site-directed mutagenesis by biolistic transformation efficiently generates inheritable mutations in a targeted locus in soybean somatic embryos and transgene-free descendants in the T
Antigens, Plant
/ adverse effects
Biolistics
CRISPR-Cas Systems
/ genetics
Crops, Agricultural
/ genetics
Gene Editing
Genome, Plant
Humans
Mutagenesis, Site-Directed
Mutation
/ genetics
Plant Breeding
Plants, Genetically Modified
/ genetics
Soybean Proteins
/ adverse effects
Glycine max
/ genetics
Transgenes
/ genetics
CRISPR/Cas9
Glycine max
Nonsense mRNA decay
Null-segregant
Transgenic embryo
Journal
Transgenic research
ISSN: 1573-9368
Titre abrégé: Transgenic Res
Pays: Netherlands
ID NLM: 9209120
Informations de publication
Date de publication:
02 2021
02 2021
Historique:
received:
20
08
2020
accepted:
04
12
2020
pubmed:
3
1
2021
medline:
28
12
2021
entrez:
2
1
2021
Statut:
ppublish
Résumé
The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated endonuclease 9 (Cas9) system is being rapidly developed for mutagenesis in higher plants. Ideally, foreign DNA introduced by this system is removed in the breeding of edible crops and vegetables. Here, we report an efficient generation of Cas9-free mutants lacking an allergenic gene, Gly m Bd 30K, using biolistic transformation and the CRISPR/Cas9 system. Five transgenic embryo lines were selected on the basis of hygromycin resistance. Cleaved amplified polymorphic sequence analysis detected only two different mutations in e all of the lines. These results indicate that mutations were induced in the target gene immediately after the delivery of the exogenous gene into the embryo cells. Soybean plantlets (T
Identifiants
pubmed: 33386504
doi: 10.1007/s11248-020-00229-4
pii: 10.1007/s11248-020-00229-4
doi:
Substances chimiques
Antigens, Plant
0
Gly m BD 30K protein, Glycine max
0
Soybean Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
77-89Références
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