Harnessing CRISPR-Cas9 for Genome Editing in Streptococcus pneumoniae D39V.


Journal

Applied and environmental microbiology
ISSN: 1098-5336
Titre abrégé: Appl Environ Microbiol
Pays: United States
ID NLM: 7605801

Informations de publication

Date de publication:
26 02 2021
Historique:
received: 10 11 2020
accepted: 21 12 2020
pubmed: 6 1 2021
medline: 7 4 2021
entrez: 5 1 2021
Statut: epublish

Résumé

CRISPR-Cas systems provide bacteria and archaea with adaptive immunity against viruses and plasmids by the detection and cleavage of invading foreign DNA. Modified versions of this system can be exploited as a biotechnological tool for precise genome editing at a targeted locus. Here, we developed a replicative plasmid that carries the CRISPR-Cas9 system for RNA-programmable genome editing by counterselection in the opportunistic human pathogen

Identifiants

pubmed: 33397704
pii: AEM.02762-20
doi: 10.1128/AEM.02762-20
pmc: PMC8105017
pii:
doi:

Substances chimiques

Bacterial Proteins 0
Virulence Factors 0
CRISPR-Associated Protein 9 EC 3.1.-

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

Copyright © 2021 Synefiaridou and Veening.

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Auteurs

Dimitra Synefiaridou (D)

Department of Fundamental Microbiology, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland.

Jan-Willem Veening (JW)

Department of Fundamental Microbiology, Faculty of Biology and Medicine, University of Lausanne, Lausanne, Switzerland Jan-Willem.Veening@unil.ch.

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Classifications MeSH