Regulation of las and rhl Quorum Sensing on Aerobic Denitrification in Pseudomonas aeruginosa PAO1.
Journal
Current microbiology
ISSN: 1432-0991
Titre abrégé: Curr Microbiol
Pays: United States
ID NLM: 7808448
Informations de publication
Date de publication:
Feb 2021
Feb 2021
Historique:
received:
11
03
2020
accepted:
14
12
2020
pubmed:
6
1
2021
medline:
15
5
2021
entrez:
5
1
2021
Statut:
ppublish
Résumé
The bacterium Pseudomonas aeruginosa negatively regulates denitrification under anerobic conditions by two acyl-homoserine lactone quorum-sensing (QS) systems called las and rhl. However, it is unknown whether these systems have the same effect on denitrification in aerobic conditions. In this study, we investigated the regulation of las and rhl systems on aerobic denitrification. We showed that the removal of nitrate in P. aeruginosa PAO1 was repressed by both the las and rhl systems. The las and rhl systems had negative effects on activities of denitrifying enzymes NAP, NIR, NOR, and NOS. At the level of transcription, both QS systems inhibited the expression of target genes napA, nirS, norB, norC, and nosZ. Furthermore, the addition of an acylase, which degrades the acyl-homoserine lactone signals (AHLs), to wild type resulted in an increase in the removal of nitrate. Additionally, in aerobic denitrification process, the transcription factor DNR, which controls denitrification, was repressed by both QS systems. The results implied that modulation of QS in denitrifying bacteria, possibly through quorum quenching or QS inhibition, could help to improve the reduction of nitrate in wastewater treatment.
Identifiants
pubmed: 33398446
doi: 10.1007/s00284-020-02338-z
pii: 10.1007/s00284-020-02338-z
doi:
Substances chimiques
Bacterial Proteins
0
Transcription Factors
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
659-667Subventions
Organisme : Natural Science Foundation of Zhejiang Province
ID : LY17E080001
Organisme : Natural Science Foundation of Zhejiang Province
ID : LQ18E080005
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