Preparative isolation of 1,1-diphenyl-2-picrylhydrazyl inhibitors from Ribes himalense using medium-pressure and two-dimensional reversed-phase/reversed-phase liquid chromatography guided by an online HPLC-1, 1-diphenyl-2-picrylhydrazyl assay.
Ribes himalense
free radical inhibitors
liquid chromatography
medium-pressure chromatography
Journal
Journal of separation science
ISSN: 1615-9314
Titre abrégé: J Sep Sci
Pays: Germany
ID NLM: 101088554
Informations de publication
Date de publication:
Apr 2021
Apr 2021
Historique:
revised:
25
12
2020
received:
19
10
2020
accepted:
01
01
2021
pubmed:
6
1
2021
medline:
26
10
2021
entrez:
5
1
2021
Statut:
ppublish
Résumé
The lack of suitable chromatographic purification methods makes it a challenge to effectively isolate the chemical components of traditional Tibetan medicines. Ribes himalense is a rarely studied Tibetan medicine, reputed to have free radical-scavenging effects. In the present work, we utilized it as a model herb to highlight an approach for the separation of 1,1-diphenyl-2-picrylhydrazyl inhibitors via medium-pressure chromatography and two-dimensional reversed-phase/reversed-phase interaction liquid chromatography under the guidance of an online high-performance liquid chromatography-1,1-diphenyl-2-picrylhydrazyl assay. Finally, we obtained two free radical inhibitors (>95% purity) from the R. himalense extract. This is the first report of the rapid isolation of these free radical inhibitors from R. himalense. This method can be useful in quality standard assessment and further pharmacological activity research, and may be used as a reference for the composition research of various natural products.
Identifiants
pubmed: 33400375
doi: 10.1002/jssc.202001080
doi:
Substances chimiques
Biphenyl Compounds
0
Picrates
0
Plant Extracts
0
1,1-diphenyl-2-picrylhydrazyl
DFD3H4VGDH
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1345-1352Subventions
Organisme : Major New Drug Creation Special Projects
ID : 2019ZX09201001
Informations de copyright
© 2021 Wiley-VCH GmbH.
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