tRNA 3' shortening by LCCR4 as a response to stress in Trypanosoma brucei.
Journal
Nucleic acids research
ISSN: 1362-4962
Titre abrégé: Nucleic Acids Res
Pays: England
ID NLM: 0411011
Informations de publication
Date de publication:
22 02 2021
22 02 2021
Historique:
accepted:
19
12
2020
revised:
24
11
2020
received:
29
04
2020
pubmed:
7
1
2021
medline:
4
3
2021
entrez:
6
1
2021
Statut:
ppublish
Résumé
Sensing of environmental cues is crucial for cell survival. To adapt to changes in their surroundings cells need to tightly control the repertoire of genes expressed at any time. Regulation of translation is key, especially in organisms in which transcription is hardly controlled, like Trypanosoma brucei. In this study, we describe the shortening of the bulk of the cellular tRNAs during stress at the expense of the conserved 3' CCA-tail. This tRNA shortening is specific for nutritional stress and renders tRNAs unsuitable substrates for translation. We uncovered the nuclease LCCR4 (Tb927.4.2430), a homologue of the conserved deadenylase Ccr4, as being responsible for tRNA trimming. Once optimal growth conditions are restored tRNAs are rapidly repaired by the trypanosome tRNA nucleotidyltransferase thus rendering the recycled tRNAs amenable for translation. This mechanism represents a fast and efficient way to repress translation during stress, allowing quick reactivation with a low energy input.
Identifiants
pubmed: 33406257
pii: 6066639
doi: 10.1093/nar/gkaa1261
pmc: PMC7897491
doi:
Substances chimiques
RNA, Transfer
9014-25-9
Ribonucleases
EC 3.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1647-1661Informations de copyright
© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.
Références
Methods Enzymol. 2007;425:21-53
pubmed: 17673078
Nat Struct Mol Biol. 2019 Sep;26(9):783-791
pubmed: 31439941
RNA. 2020 Feb;26(2):199-208
pubmed: 31719125
J Biol Chem. 1958 Mar;231(1):241-57
pubmed: 13538965
Cell Rep. 2019 Mar 19;26(12):3416-3428.e5
pubmed: 30893612
Biochim Biophys Acta. 2013 Jun-Jul;1829(6-7):571-9
pubmed: 23274303
RNA Biol. 2020 Aug;17(8):1057-1059
pubmed: 32715957
Wiley Interdiscip Rev RNA. 2017 Sep;8(5):
pubmed: 28608481
Nucleic Acids Res. 2020 Jan 8;48(D1):D1145-D1152
pubmed: 31686107
EMBO J. 2002 Mar 15;21(6):1427-36
pubmed: 11889048
BMC Genomics. 2008 Jan 03;9:2
pubmed: 18173843
FEBS Lett. 2009 Jan 22;583(2):437-42
pubmed: 19114040
Gene. 1988 Dec 10;72(1-2):75-89
pubmed: 2468576
Mol Cell Biol. 2005 Aug;25(16):7303-13
pubmed: 16055738
Nature. 1974 Aug 16;250(467):546-51
pubmed: 4602655
J Biol Chem. 2019 Nov 8;294(45):16930-16941
pubmed: 31582561
Biochimie. 2008 May;90(5):762-72
pubmed: 18226598
J Biol Chem. 1996 Aug 2;271(31):18638-42
pubmed: 8702516
J Biol Chem. 1990 Sep 25;265(27):16216-20
pubmed: 2204621
Nat Commun. 2019 Jan 10;10(1):118
pubmed: 30631057
Biosci Biotechnol Biochem. 2016 Jun;80(6):1037-45
pubmed: 26967967
Cell. 2014 Sep 11;158(6):1281-1292
pubmed: 25215487
Trends Genet. 2012 Nov;28(11):574-81
pubmed: 22921354
J Cell Biol. 2009 Apr 6;185(1):43-50
pubmed: 19332891
Open Biol. 2019 Jun 28;9(6):190072
pubmed: 31164043
Biochim Biophys Acta Gene Regul Mech. 2018 Apr;1861(4):373-386
pubmed: 29191733
Wiley Interdiscip Rev RNA. 2011 May-Jun;2(3):362-75
pubmed: 21572561
Nat Protoc. 2011 May;6(5):580-92
pubmed: 21527916
Cold Spring Harb Perspect Biol. 2019 May 1;11(5):
pubmed: 30082464
Mol Biochem Parasitol. 1999 Mar 15;99(1):89-101
pubmed: 10215027
Elife. 2018 Mar 15;7:
pubmed: 29543155
Cell Rep. 2020 Feb 18;30(7):2106-2114.e5
pubmed: 32075755
Nucleic Acids Res. 1987 Nov 11;15(21):8783-98
pubmed: 3684574
Proc Natl Acad Sci U S A. 1987 Apr;84(8):2238-41
pubmed: 3470787
Mol Cell. 2018 Jun 21;70(6):1089-1100.e8
pubmed: 29932902
Curr Biol. 2003 Feb 4;13(3):189-98
pubmed: 12573214
J Biol Chem. 2020 May 1;295(18):6138-6150
pubmed: 32234763
Int J Med Microbiol. 2012 Oct;302(4-5):221-4
pubmed: 22964417
J Biol Chem. 2002 Sep 6;277(36):32849-54
pubmed: 12095995
Nucleic Acids Res. 2017 Sep 29;45(17):10306-10320
pubmed: 28973472
PLoS Genet. 2013 Aug;9(8):e1003767
pubmed: 24009533
EMBO J. 2010 Aug 4;29(15):2566-76
pubmed: 20628353
J Mol Biol. 1977 Feb 5;109(4):593-7
pubmed: 321788
Nucleic Acids Res. 2017 Jan 25;45(2):793-804
pubmed: 27903898
Curr Opin Struct Biol. 2006 Feb;16(1):12-7
pubmed: 16364630
Curr Opin Microbiol. 2007 Dec;10(6):539-46
pubmed: 17997129
Science. 2020 Jul 31;369(6503):524-530
pubmed: 32732418
Wiley Interdiscip Rev RNA. 2016 Jul;7(4):438-54
pubmed: 26821858
J Cell Biol. 2009 Apr 6;185(1):35-42
pubmed: 19332886
Nat Struct Mol Biol. 2019 May;26(5):343-349
pubmed: 31011209
J Biol Chem. 2004 Apr 2;279(14):13616-23
pubmed: 14734555
Bioessays. 2019 May;41(5):e1900009
pubmed: 31026340
Cell. 2001 Feb 9;104(3):377-86
pubmed: 11239395
Nucleic Acids Res. 2008 Jun;36(10):3374-88
pubmed: 18442996
Front Genet. 2014 Jan 02;4:299
pubmed: 24427169
J Biol Chem. 1972 Aug 25;247(16):5243-51
pubmed: 4560501
J Eukaryot Microbiol. 2012 Sep;59(5):429-93
pubmed: 23020233
J Biol Chem. 2009 Dec 25;284(52):36491-9
pubmed: 19875444
Biochim Biophys Acta. 2013 Jun-Jul;1829(6-7):561-70
pubmed: 23337855
J Biol Chem. 2004 Jun 4;279(23):23988-95
pubmed: 15044470