Diagnosis and identification of Leishmania species in patients with cutaneous leishmaniasis in the state of Roraima, Brazil's Amazon Region.
Adolescent
Adult
Brazil
/ epidemiology
Child
Child, Preschool
DNA, Protozoan
/ genetics
Epidemiological Monitoring
Female
Humans
Infant
Infant, Newborn
Leishmania
/ classification
Leishmania braziliensis
/ genetics
Leishmania guyanensis
/ genetics
Leishmaniasis, Cutaneous
/ diagnosis
Male
Middle Aged
Prospective Studies
Sequence Analysis, DNA
Young Adult
Cutaneous leishmaniasis
Leishmania spp.
Molecular diagnosis
PCR
Journal
Parasites & vectors
ISSN: 1756-3305
Titre abrégé: Parasit Vectors
Pays: England
ID NLM: 101462774
Informations de publication
Date de publication:
07 Jan 2021
07 Jan 2021
Historique:
received:
27
06
2020
accepted:
11
12
2020
entrez:
8
1
2021
pubmed:
9
1
2021
medline:
22
7
2021
Statut:
epublish
Résumé
Cutaneous leishmaniasis (CL) is an endemic disease in Brazil that is highly prevalent in the northern region of the country. Although there is a continuous and growing number of cases registered in the state of Roraima, there is limited information regarding the species of Leishmania that affect the human population. In this study, we aimed to characterize which Leishmania species cause human disease in those presenting with cutaneous leishmaniasis in endemic areas of the State of Roraima. We conducted a prospective surveillance study between 2016 to 2018 in health centers located in the State of Roraima, Brazil. Participants with clinical suspicion of CL were enrolled and provided lesion samples for parasitological detection by microscopy. A subset of the samples was tested by polymerase chain reaction and sequencing of the internal transcribed spacer 1 (ITS-1 PCR) for molecular species identification. A total of 262 participants were enrolled in this study. Of those, 129 (49.27%) were positive by parasitological examination. Most positive subjects (81.58%) were male, and most cases presented a single lesion (80.26%). ITS-1 PCR and sequencing on a subset of 76 samples allowed us to detect nine different species of Leishmania: L. (V.) braziliensis, L (V.) panamensis, L. (V.) guyanensis, L. (V.) naiffi, L. (V.) shawi, L.(V.) utingensis, L. (V.) lindenbergi, L. (L.) amazonensis and L. (L.) mexicana. Our study provides the first assessment of circulating species of Leishmania in the State of Roraima, Brazil, and shows the high diversity in this region. This study opens the path for further research on the transmission of leishmaniasis in the northernmost Brazilian State including vector and reservoir surveillance as well as for intensification of investigation and control activities against CL in the region.
Sections du résumé
BACKGROUND
BACKGROUND
Cutaneous leishmaniasis (CL) is an endemic disease in Brazil that is highly prevalent in the northern region of the country. Although there is a continuous and growing number of cases registered in the state of Roraima, there is limited information regarding the species of Leishmania that affect the human population. In this study, we aimed to characterize which Leishmania species cause human disease in those presenting with cutaneous leishmaniasis in endemic areas of the State of Roraima.
METHODS
METHODS
We conducted a prospective surveillance study between 2016 to 2018 in health centers located in the State of Roraima, Brazil. Participants with clinical suspicion of CL were enrolled and provided lesion samples for parasitological detection by microscopy. A subset of the samples was tested by polymerase chain reaction and sequencing of the internal transcribed spacer 1 (ITS-1 PCR) for molecular species identification.
RESULTS
RESULTS
A total of 262 participants were enrolled in this study. Of those, 129 (49.27%) were positive by parasitological examination. Most positive subjects (81.58%) were male, and most cases presented a single lesion (80.26%). ITS-1 PCR and sequencing on a subset of 76 samples allowed us to detect nine different species of Leishmania: L. (V.) braziliensis, L (V.) panamensis, L. (V.) guyanensis, L. (V.) naiffi, L. (V.) shawi, L.(V.) utingensis, L. (V.) lindenbergi, L. (L.) amazonensis and L. (L.) mexicana.
CONCLUSIONS
CONCLUSIONS
Our study provides the first assessment of circulating species of Leishmania in the State of Roraima, Brazil, and shows the high diversity in this region. This study opens the path for further research on the transmission of leishmaniasis in the northernmost Brazilian State including vector and reservoir surveillance as well as for intensification of investigation and control activities against CL in the region.
Identifiants
pubmed: 33413563
doi: 10.1186/s13071-020-04539-8
pii: 10.1186/s13071-020-04539-8
pmc: PMC7791761
doi:
Substances chimiques
DNA, Protozoan
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
32Subventions
Organisme : CAPES
ID : 1647/2018
Organisme : Armed Forces Health Surveillance Branch
ID : P0143_19_N6_03.
Références
Brasil. Ministério da Saúde. Secretaria de Vigilância em Saúde. Departamento de Vigilância das Doenças Transmissíveis. Manual de vigilância da leishmaniose tegumentar. Secretaria de Vigilância em Saúde, Departamento de Vigilância das Doenças Transmissíveis. Brasília, 2017, p. 189.
WHO. Leishmaniasis: epidemiological situation. World Health Organization. https://www.who.int/leishmaniasis/burden/en/ . Accessed Mar 2020.
Almeida JV, Brazil RP. Retrospective study of cutaneous leishmaniasis in The State of Roraima, North of Brazil. Journal of Dermatology and Clinical Research 2017.
Negrão GN, Ferreira MEM. Considerações sobre a leishmaniose tegumentar americana e sua expansão no território brasileiro. Revista Percurso NEMO. 2014;1(6):147–68.
doi: 10.4025/revpercurso.v6i1.21375
Athias R, Machado MA. Saúde indígena no processo de implantação dos Distritos Sanitários: temas críticos e propostas para um diálogo interdisciplinar. Cad Saúde Pública. 2001;17(2):425–31.
doi: 10.1590/S0102-311X2001000200017
Gama Neto JL, Baima JM, Freitas RA, Passos MAB. Fauna flebotomínica (Diptera: Psychodidae) da Serra do Tepequém, Município de Amajarí, Estado de Roraima, Brasil. Rev Pan-Amaz Saude. 2010;1(2):131–6.
doi: 10.5123/S2176-62232010000200016
Brasil. Ministério da Saúde. Leishmaniose Tegumentar Americana – Casos confirmados Notificados no Sistema de Informação de Agravos de Notificação-SINAN EM Roraima entre os anos 2016 a 2018. www.saúde.gov.br.
Pezente LG, Benedetti MSG. Perfil epidemiológico da leishmaniose Tegumentar Americana no Estado de Roraima, Amazônia, Brasil, entre 2007 e 2016. Braz J Hea Rev 2019;2(3):1734–1742. Accessed Aug 2020.
Brasil. Constituição de 1988. Constituição da República Federativa do Brasil. Brasília: Supremo Tribunal Federal, Secretaria de Documentação, 2019, p. 577. Atualizada até a EC n. 105/2019. ISBN: 978-85-54223-41-0. www.stf.jus.br/arquivo/cms/legislacaoConstituicao/anexo/CF.pde . Accessed Sept 2019.
IBGE. Instituto Brasileiro de Geografia e Estatística. Estimativa populacional do Estado de Roraima, 2019. https://www.ibge.gov.br/pt/inicio.html . Accessed Sept 2019.
Schonian G, Nasereddin A, Dinse N, Schweynoch C, Schallig HD, Presber W, et al. Diagnóstico por PCR e caracterização de Leishmania em amostras clínicas locais e importadas. Diagn Microbiol Infect Dis. 2003;47:349–58.
doi: 10.1016/S0732-8893(03)00093-2
GenBank. International nucleotide sequence database. https://www.ncbi.nlm.nih.gov/genbank/ . Accessed June 2020.
Sievers F, Wilm A, Dineen D, Gibson TJ, Karplus K, Li W, et al. Fast, scalable generation of high-quality protein multiple sequence alignments using Clustal Omega. Mol Syst Biol. 2011;7(539):1–6.
Paradis E, Schliep K. Ape 5.0: an environment for modern phylogenetics and evolutionary analyses in R. Bioinformatics. 2019;35(3):526–8.
doi: 10.1093/bioinformatics/bty633
Kamvar ZN, Tabima JF, Grünwald NJ. Poppr: an R package for genetic analysis of populations with clonal, partially clonal, and/or sexual reproduction. PeerJ. 2014;2:e281.
doi: 10.7717/peerj.281
Jombart T. Aegenet: um pacote R para a análise multivariada de marcadores genéticos. Bioinformática. 2008;24(11):1403–5.
doi: 10.1093/bioinformatics/btn129
BioEstat 5.0. Download de Arquivos. http://www.mamiraua.org.br/download/index.php?dirpath=./BioEstat%205%20Portugues&order=0 . Accessed Sept 2019.
Sampaio RNR, Gonçalves MC, Leite VA, França BV, Santos G, Carvalho MSL, et al. Estudo da transmissão da leishmaniose tegumentar americana no Distrito Federal. Rev Soc Bras Med Trop. 2009;42(6):686–90.
doi: 10.1590/S0037-86822009000600015
Coelho LIC, Paes M, Guerra JA, Barbosa MG, Coelho C, Lima B, et al. Characterization of Leishmania spp. Causing cutaneous leishmaniasis in Manaus, Amazonas, Brazil. Parasitol Res. 2011;108:671–7.
doi: 10.1007/s00436-010-2139-9
Naiff Júnior RD, Pinheiro FG, Naiff MF, Souza IS, Castro LM, Menezes MP, et al. Estudo de uma série de casos de Leishmaniose Tegumentar Americana no município de Rio Preto da Eva, Amazonas Brasil. Revista de Patologia Tropical. 2009;38:103–4.
Bacha HA, Tuon FF, Zampieri RA, Floeter-Winter LM, Oliveira J, Nicodemo AC, et al. Leishmania(Viannia) braziliensis identificação por PCR no estado do Pará Brasil. Transações da Royal Society of Tropical Medicine and Hygiene. 2011;105(3):173–8.
doi: 10.1016/j.trstmh.2010.11.010
Araujo-Pereira T, Pereia DP, Moreira RB, Galdino TS, Duarte MPO, Brazil RP, et al. Molecular diagnosis of cutaneous leishmaniasis in an endemic area of Acre State in the Amazonian Region of Brazil. Rev Soc Bras Med Trop. 2018;51(3):376–81.
doi: 10.1590/0037-8682-0232-2017
Da Silva ACT, Cupolillo E, Volpini AC, Almeida R, Romero GA. Species diversity causing human cutaneous leishmaniasis in Rio Branco, state of Acre, Brazil. Trop Med Int Health. 2006;11(9):1388–98.
doi: 10.1111/j.1365-3156.2006.01695.x
Gama Neto JL, Baima JM, Freitas RA, Passos MAB. Fauna flebotomínica (Diptera: Psychodidae) em floresta preservada e alterada do Município de Caroebe, Estado de Roraima, Brasil. Rev Pan-Amaz Saude. 2012;3(2):41–6.
doi: 10.5123/S2176-62232012000200006
Souza AAA, Silveira FT, Lainson R, Barata IR, Silva MGS, Lima JAN. Fauna flebotomínica da Serra dos Carajás, Estado do Pará, Brasil, e sua possível implicação na transmissão da leishmaniose tegumentar americana. Rev Pan-Amaz Saude. 2010;1(1):45–51.
doi: 10.5123/S2176-62232010000100007
Barral A, Pedral-Sampaio D, Junior GG, Moem H, McMahon-Pratt D, Jesus AR, et al. Leishmaniasis in Bahia, Brazil: evidence that Leishmania amazonensis produces a wide spectrum of clinical disease. Am J Trop Med Hyg. 1991;44:536–46.
doi: 10.4269/ajtmh.1991.44.536
Lainson R, Shaw JJ. Taxonomy of the New World Leishmania species. Trans R Soc Trop Med Hyg. 1972;66(6):943–4.
doi: 10.1016/0035-9203(72)90133-2
Lainson R. Espécies neotropicais de Leishmania: uma breve revisão histórica sobre sua descoberta, ecologia e taxonomia. Rev Pan-Amaz Saude. 2010;1(2):13–32.
doi: 10.5123/S2176-62232010000200002
Guerra JAdO, Prestes SR, Silveira H, Coelho LIdARC, Gama P, Moura A, et al. Mucosal Leishmaniasis Caused by Leishmania (Viannia) braziliensis and Leishmania (Viannia) guyanensis in the Brazilian Amazon. PLoS Negl Trop Dis. 2011;5(3):e980.
doi: 10.1371/journal.pntd.0000980
Vásquez A, Paz H, Alvar J, Pérez D, Hernández C. Informe Final: Estudos Sobre a Epidemiologia da Leishmaniose na Parte Ocidental da República do Panamá. Ministério da Saúde: Instituto Comemorativo Gorgas de Estúdio da Saúde; 1998.
Davies CR, Reithinger R, Campbell-Lendrum D, Feliciangeli D, Borges R, Rodriguez N. Epidemiologia e controle da leishmaniose nos países andinos Cafajeste. Saude Publica. 2000;16:925–50.
doi: 10.1590/S0102-311X2000000400013
Lainson R, Braga RR, Souza AA, Povoa MM, Ishikawa EA, Silveira FT. Leishmania (Viannia) shawi sp. n., a parasite of monkeys, sloths and procyonids in Amazonian Brazil. Ann Parasitol Hum Comp. 1989;64(3):200–7.
doi: 10.1051/parasite/1989643200
Figueira LP, Zanotti M, Pinheiro FG, Franco AMR. Caracterização isoenzimática de isolados humanos de Leishmania sp (Kinetoplastida: Trypanosomatidae) dos municípios de Rio Preto da Eva e Manaus, Estado do Amazonas. Rev Soc Bras Med Trop. 2008;41(5):512–4.
doi: 10.1590/S0037-86822008000500016
Lainson R. Espécies neotropicais de Leishmania: uma breve revisão histórica sobre sua descoberta, ecologia e taxonomia. Revista Pan-Amazônica de Saúde. 2010;1(2):13–32.
doi: 10.5123/S2176-62232010000200002
Fagundes-Silva GA, Romero GAS, Cupolillo E, Yamashita EPG, Gomes-Silva A, de Guerra JAO, et al. Leishmania (Viannia) naiffi: rare enough to be neglected? Mem Inst Oswaldo Cruz. 2015;110(6):797–800.
doi: 10.1590/0074-02760150128
Braga RR, Lainson R, Ishikawa EAY, Shaw JJ. Leishmania (Viannia) utingensis n.sp., a parasite from the sandfly Lutzomyia (Viannamyia) tuberculata in Amazonian Brazil. Parasite. 2003;10:111–8.
doi: 10.1051/parasite/2003102111
Cantanhêde LM, Mattos CB, Ronconi CS, Filgueira CPB, Júnior CFS, Limeira C, et al. First report of Leishmania (Viannia) lindenbergi causing tegumentary leishmaniasis in the Brazilian western Amazon region. Parasite. 2019;26:30.
doi: 10.1051/parasite/2019030
Silveira FT, Ishikawa EAI, De Souza AAA, Lainson R. An outbreak of cutaneous leishmaniasis among soldiers in Belém, Pará State, Brazil caused by Leishmania (Viannia) lindenbergi n. sp., a new leishmanial parasite of man in the Amazon region. Parasite. 2002;9:43–50.
doi: 10.1051/parasite/200209143