Bacteriocin isolated from the natural inhabitant of Allium cepa against Staphylococcus aureus.
Animals
Anti-Bacterial Agents
/ pharmacology
Antimicrobial Cationic Peptides
/ pharmacology
Bacillus
Bacteriocins
/ isolation & purification
HEK293 Cells
Humans
Hydrogen-Ion Concentration
Kinetics
Methicillin-Resistant Staphylococcus aureus
/ drug effects
Mice
Microbial Sensitivity Tests
Multigene Family
Onions
/ chemistry
Plant Extracts
/ pharmacology
RAW 264.7 Cells
Staphylococcal Infections
Staphylococcus aureus
/ drug effects
Temperature
Whole Genome Sequencing
Antimicrobial peptides (AMPs)
DiBAC4 (3)
MRSA
Propidium iodide
Staphylococcus aureus
Journal
World journal of microbiology & biotechnology
ISSN: 1573-0972
Titre abrégé: World J Microbiol Biotechnol
Pays: Germany
ID NLM: 9012472
Informations de publication
Date de publication:
11 Jan 2021
11 Jan 2021
Historique:
received:
14
05
2020
accepted:
22
12
2020
entrez:
11
1
2021
pubmed:
12
1
2021
medline:
9
7
2021
Statut:
epublish
Résumé
Extensive usage of antibiotics has led to the emergence of drug-resistant strains of pathogens and hence, there is an urgent need for alternative antimicrobial agents. Antimicrobial Peptides (AMPs) of bacterial origin have shown the potential to replace some conventional antibiotics. In the present study, an AMP was isolated from Bacillus subtilis subsp. spizizenii strain Ba49 present on the Allium cepa, the common onion and named as peptide-Ba49. The isolated AMP was purified and characterized. The purified peptide-Ba49, having a molecular weight of ~ 3.3 kDa as determined using mass spectroscopy, was stable up to 121 °C and in the pH range of 5-10. Its interaction with protein degrading enzymes confirmed the peptide nature of the molecule. The peptide exhibited low minimum inhibitory concentration (MIC) against Staphylococcus aureus and its (Methicillin-resistant Staphylococcus aureus) MRSA strains (MIC, 2-16 µM/mL). Further, time kill kinetic assay was performed and analysis of the results of membrane depolarization and permeabilization assays (TEM, DiBAC
Identifiants
pubmed: 33427970
doi: 10.1007/s11274-020-02989-x
pii: 10.1007/s11274-020-02989-x
doi:
Substances chimiques
Anti-Bacterial Agents
0
Antimicrobial Cationic Peptides
0
Bacteriocins
0
Plant Extracts
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
20Références
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