Dichroic Fourier Transform Infrared Spectroscopy Characterization of the β-Sheet Orientation in Spider Silk Films on Silicon Substrates.

Protein Conformation, beta-Strand Recombinant Proteins Silicon Silk Spectroscopy, Fourier Transform Infrared

Journal

The journal of physical chemistry. B
ISSN: 1520-5207
Titre abrégé: J Phys Chem B
Pays: United States
ID NLM: 101157530

Informations de publication

Date de publication:
04 02 2021
Historique:
pubmed: 13 1 2021
medline: 15 5 2021
entrez: 12 1 2021
Statut: ppublish

Résumé

Orientation analysis of the β-sheet structure within films of the established recombinant spider silk protein eADF4(C16) was performed using a concept based on dichroic transmission- and attenuated total reflection-Fourier transform infrared spectroscopy, lineshape analysis, assignment of amide I components to specific vibration modes, and transition dipole moment directions of β-sheet structures. Based on the experimental dichroic ratio

Identifiants

DOI: 10.1021/acs.jpcb.0c09395 PMID: 33433229
pubmed: 33433229
doi: 10.1021/acs.jpcb.0c09395
doi:

Substances chimiques

Recombinant Proteins 0
Silk 0
Silicon Z4152N8IUI

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1061-1071

Auteurs

Mirjam Hofmaier (M)

Institute of Physical Chemistry and Polymer Physics, Leibniz Institute of Polymer Research Dresden (IPF), Hohe Strasse 6, D-01069 Dresden, Germany.
Chair of Macromolecular Chemistry, Technical University of Dresden (TUD), Mommsenstraße 4, D-01062 Dresden, Germany.

Birgit Urban (B)

Institute of Physical Chemistry and Polymer Physics, Leibniz Institute of Polymer Research Dresden (IPF), Hohe Strasse 6, D-01069 Dresden, Germany.

Sarah Lentz (S)

Chair of Biomaterials, University of Bayreuth, Prof.-Rüdiger-Bormann-Str. 1, D-95447 Bayreuth, Germany.

Christian B Borkner (CB)

Chair of Biomaterials, University of Bayreuth, Prof.-Rüdiger-Bormann-Str. 1, D-95447 Bayreuth, Germany.

Thomas Scheibel (T)

Chair of Biomaterials, University of Bayreuth, Prof.-Rüdiger-Bormann-Str. 1, D-95447 Bayreuth, Germany.

Andreas Fery (A)

Institute of Physical Chemistry and Polymer Physics, Leibniz Institute of Polymer Research Dresden (IPF), Hohe Strasse 6, D-01069 Dresden, Germany.
Chair of Physical Chemistry of Polymeric Materials, Technical University Dresden (TUD), D-01069 Dresden, Germany.

Martin Müller (M)

Institute of Physical Chemistry and Polymer Physics, Leibniz Institute of Polymer Research Dresden (IPF), Hohe Strasse 6, D-01069 Dresden, Germany.
Chair of Macromolecular Chemistry, Technical University of Dresden (TUD), Mommsenstraße 4, D-01062 Dresden, Germany.
ORCID: 0000-0001-8961-4604

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Classifications MeSH

questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Structure secondaire des protéines › Structure en brin bêta › Dichroic Fourier Transform Infrared...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines recombinantes › Dichroic Fourier Transform Infrared...
questionsmedicales.fr › Produits chimiques inorganiques › Éléments › Métalloïdes › Silicium › Dichroic Fourier Transform Infrared...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines d'arthropode › Soie › Dichroic Fourier Transform Infrared...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Analyse spectrale › Spectrophotométrie › Spectrophotométrie IR › Spectroscopie infrarouge à transformée de Fourier › Dichroic Fourier Transform Infrared...