Trypanosoma brucei transferrin receptor: Functional replacement of the GPI anchor with a transmembrane domain.
Base Sequence
Cell Membrane
/ chemistry
Cloning, Molecular
Escherichia coli
/ genetics
Gene Expression
Glycosylation
Glycosylphosphatidylinositols
/ chemistry
Plasmids
/ chemistry
Protein Domains
Protein Engineering
Protein Multimerization
Protein Processing, Post-Translational
Protein Subunits
/ chemistry
Protozoan Proteins
/ chemistry
Receptors, Transferrin
/ chemistry
Recombinant Proteins
/ chemistry
Trypanosoma brucei brucei
/ genetics
GPI-anchor
Transferrin receptor
Transmembrane domain
Trypanosoma brucei
Journal
Molecular and biochemical parasitology
ISSN: 1872-9428
Titre abrégé: Mol Biochem Parasitol
Pays: Netherlands
ID NLM: 8006324
Informations de publication
Date de publication:
03 2021
03 2021
Historique:
received:
19
11
2020
revised:
05
01
2021
accepted:
08
01
2021
pubmed:
16
1
2021
medline:
31
7
2021
entrez:
15
1
2021
Statut:
ppublish
Résumé
The transferrin receptor of Trypanosoma brucei (TbTfR) is a heterodimer of a glycosylphosphatidylinositol (GPI)-anchored ESAG6 subunit and an ESAG7 subunit. To investigate whether the GPI-anchor is essential for the function of the TbTfR, an ESAG6 with a transmembrane domain instead of a GPI-anchor (ESAG6tmd) was inducibly expressed in bloodstream form trypanosomes. It is shown that the ESAG6tmd is able to dimerise with ESAG7 to form a TbTfR that can bind transferrin. Fractionation experiments clearly demonstrated that the transmembrane-anchored TbTfR is exclusively associated with the membrane fraction. No difference in the uptake of transferrin was observed between trypanosomes inducibly expressing a transmembrane-anchored TbTfR and trypanosomes inducibly expressing a GPI-anchored TbTfR. Differences in glycosylation pattern of ESAG6tmd and native ESAG6 may indicate different intracellular trafficking of transmembrane- and GPI-anchored TbTfRs. The findings suggest that the GPI-anchor is not essential for the function of the TbTfR in bloodstream forms of T. brucei.
Identifiants
pubmed: 33450336
pii: S0166-6851(21)00012-8
doi: 10.1016/j.molbiopara.2021.111361
pii:
doi:
Substances chimiques
Glycosylphosphatidylinositols
0
Protein Subunits
0
Protozoan Proteins
0
Receptors, Transferrin
0
Recombinant Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
111361Informations de copyright
Copyright © 2021 Elsevier B.V. All rights reserved.