Male fertility in Arabidopsis requires active DNA demethylation of genes that control pollen tube function.
Arabidopsis
/ physiology
Arabidopsis Proteins
/ genetics
DNA Demethylation
Fertility
/ genetics
Gene Expression Regulation, Developmental
Gene Expression Regulation, Plant
Mutation
N-Glycosyl Hydrolases
/ genetics
Nuclear Proteins
/ genetics
Plants, Genetically Modified
Pollen Tube
/ growth & development
Trans-Activators
/ genetics
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
18 01 2021
18 01 2021
Historique:
received:
21
02
2020
accepted:
11
12
2020
entrez:
19
1
2021
pubmed:
20
1
2021
medline:
30
1
2021
Statut:
epublish
Résumé
Active DNA demethylation is required for sexual reproduction in plants but the molecular determinants underlying this epigenetic control are not known. Here, we show in Arabidopsis thaliana that the DNA glycosylases DEMETER (DME) and REPRESSOR OF SILENCING 1 (ROS1) act semi-redundantly in the vegetative cell of pollen to demethylate DNA and ensure proper pollen tube progression. Moreover, we identify six pollen-specific genes with increased DNA methylation as well as reduced expression in dme and dme;ros1. We further show that for four of these genes, reinstalling their expression individually in mutant pollen is sufficient to improve male fertility. Our findings demonstrate an essential role of active DNA demethylation in regulating genes involved in pollen function.
Identifiants
pubmed: 33462227
doi: 10.1038/s41467-020-20606-1
pii: 10.1038/s41467-020-20606-1
pmc: PMC7813888
doi:
Substances chimiques
Arabidopsis Proteins
0
Nuclear Proteins
0
ROS1 protein, Arabidopsis
0
Trans-Activators
0
DEMETER protein, Arabidopsis
EC 3.2.2.-
N-Glycosyl Hydrolases
EC 3.2.2.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
410Subventions
Organisme : NCI NIH HHS
ID : P30 CA045508
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM067014
Pays : United States
Organisme : Howard Hughes Medical Institute
Pays : United States
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