Development of a sensitive MEKC-LIF method for synthetic cathinones analysis.

Forensic analysis MEKC-LIF Neutral surfactant Polyethylene glycol ether surfactants Synthetic cathinones

Journal

Electrophoresis
ISSN: 1522-2683
Titre abrégé: Electrophoresis
Pays: Germany
ID NLM: 8204476

Informations de publication

Date de publication:
05 2021
Historique:
revised: 18 01 2021
received: 06 11 2020
accepted: 19 01 2021
pubmed: 23 1 2021
medline: 10 2 2022
entrez: 22 1 2021
Statut: ppublish

Résumé

Synthetic cathinones are phenylalkylamine compounds related to natural cathinone from Catha edulis leaves. Due to their sympathomimetic effects comparable to common illicit drugs, these substances are mainly drugs of abuse and constitute the second most frequently seized group of new psychoactive substances. In order to ensure their regulation and to promote public health, reliable analytical tools are required to track these substances. In the present study, we developed a CE hyphenated to laser-induced fluorescence detection method to demonstrate its suitability to perform fast and cost-effective synthetic cathinones analysis. Fourteen compounds including isobaric compounds and position isomers were selected to encompass the large panel of chemical structures. To separate the FITC-labeled analytes (presenting the same negative charge and close mass to charge ratios), MEKC separation mode was selected. Method selectivity was not suitable using common surfactants. In this context, alkyl polyethylene glycol ether surfactants were successfully used as neutral surfactant to overcome this analytical challenge. The effect of surfactant nature on separation performances and migration behaviors of the analytes was also studied. Optimal BGE composition included 75 mM borate buffer at pH 9.3 and 0.4 mM of C12E10 surfactant. Final MEKC separation conditions were proposed to analyze a large panel of synthetic cathinones. This method helped to reach a sensitivity with LOD from 0.1 to 0.4 nM (pg/mL order).

Identifiants

pubmed: 33482013
doi: 10.1002/elps.202000331
doi:

Substances chimiques

Alkaloids 0
Illicit Drugs 0
Surface-Active Agents 0
cathinone 540EI4406J

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1127-1134

Informations de copyright

© 2021 Wiley-VCH GmbH.

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Auteurs

Paul Emonts (P)

Laboratory for the Analysis of Medicines, CIRM, University of Liège, Liège, Belgium.

Anne-Catherine Servais (AC)

Laboratory for the Analysis of Medicines, CIRM, University of Liège, Liège, Belgium.

Eric Ziemons (E)

Laboratory of Pharmaceutical Analytical Chemistry, CIRM, University of Liège, Liège, Belgium.

Philippe Hubert (P)

Laboratory of Pharmaceutical Analytical Chemistry, CIRM, University of Liège, Liège, Belgium.

Marianne Fillet (M)

Laboratory for the Analysis of Medicines, CIRM, University of Liège, Liège, Belgium.

Amandine Dispas (A)

Laboratory for the Analysis of Medicines, CIRM, University of Liège, Liège, Belgium.
Laboratory of Pharmaceutical Analytical Chemistry, CIRM, University of Liège, Liège, Belgium.

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