Reversible Control of Gelatin Hydrogel Stiffness by Using DNA Crosslinkers*.
DNA
biomaterials
dynamic
hydrogels
self-assembly
Journal
Chembiochem : a European journal of chemical biology
ISSN: 1439-7633
Titre abrégé: Chembiochem
Pays: Germany
ID NLM: 100937360
Informations de publication
Date de publication:
14 05 2021
14 05 2021
Historique:
received:
20
01
2021
pubmed:
24
1
2021
medline:
15
12
2021
entrez:
23
1
2021
Statut:
ppublish
Résumé
Biomaterials with dynamically tunable properties are critical for a range of applications in regenerative medicine and basic biology. In this work, we show the reversible control of gelatin methacrylate (GelMA) hydrogel stiffness through the use of DNA crosslinkers. We replaced some of the inter-GelMA crosslinks with double-stranded DNA, allowing for their removal through toehold-mediated strand displacement. The crosslinks could be restored by adding fresh dsDNA with complementary handles to those on the hydrogel. The elastic modulus (G') of the hydrogels could be tuned between 500 and 1000 Pa, reversibly, over two cycles without degradation of performance. By functionalizing the gels with a second DNA strand, it was possible to control the crosslink density and a model ligand in an orthogonal fashion with two different displacement strands. Our results demonstrate the potential for DNA to reversibly control both stiffness and ligand presentation in a protein-based hydrogel, and will be useful for teasing apart the spatiotemporal behavior of encapsulated cells.
Identifiants
pubmed: 33484601
doi: 10.1002/cbic.202100030
doi:
Substances chimiques
Biocompatible Materials
0
Cross-Linking Reagents
0
Hydrogels
0
Methacrylates
0
Gelatin
9000-70-8
DNA
9007-49-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
1755-1760Subventions
Organisme : Arizona State University
Organisme : Air Force Office of Scientific
ID : FA9550-17-1-0053
Organisme : National Science Foundation
ID : #1914680
Informations de copyright
© 2021 Wiley-VCH GmbH.
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