A deletion in SARS-CoV-2 ORF7 identified in COVID-19 outbreak in Uruguay.
Journal
Transboundary and emerging diseases
ISSN: 1865-1682
Titre abrégé: Transbound Emerg Dis
Pays: Germany
ID NLM: 101319538
Informations de publication
Date de publication:
Nov 2021
Nov 2021
Historique:
revised:
15
01
2021
received:
08
12
2020
accepted:
21
01
2021
pubmed:
28
1
2021
medline:
26
11
2021
entrez:
27
1
2021
Statut:
ppublish
Résumé
The analysis of genetic diversity in SARS-CoV-2 is the focus of several studies, providing insights into how the virus emerged and evolves. Most common changes in SARS-CoV-2 are single or point nucleotide substitutions; meanwhile, insertions and deletions (indels) have been identified as a less frequent source of viral genetic variability. Here, we report the emergence of a 12-nucleotide deletion in ORF7a, resulting in a 4-amino acid in-frame deletion. The Δ12 variant was identified in viruses from patients of a single outbreak and represents the first report of this deletion in South American isolates. Phylogenetic analysis revealed that Δ12 strains belong to the lineage B.1.1 and clustered separated from the remaining Uruguayan strains. The ∆12 variant was detected in 14 patients of this outbreak by NGS sequencing and/or two rapid and economic methodologies: Sanger amplicon sequencing and capillary electrophoresis. The presence of strong molecular markers as the deletion described here are useful for tracking outbreaks and reveal a significant aspect of the SARS-CoV-2 evolution on the robustness of the virus to keep its functionality regardless loss of genetic material.
Identifiants
pubmed: 33501730
doi: 10.1111/tbed.14002
pmc: PMC8014828
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
3075-3082Subventions
Organisme : Comisión Sectorial de Investigación Científica
ID : SARS_CoV-2 funding
Organisme : Fundación Manuel Pérez
ID : SARS-CoV-2 project
Informations de copyright
© 2021 Wiley-VCH GmbH.
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