Tissue-engineered arterial intima model exposed to steady wall shear stresses.
Custom- designed well
F-Actin
In-vitro model
Monolayer
Multi-cellular co-culture
VE-cadherin
Journal
Journal of biomechanics
ISSN: 1873-2380
Titre abrégé: J Biomech
Pays: United States
ID NLM: 0157375
Informations de publication
Date de publication:
05 03 2021
05 03 2021
Historique:
received:
29
12
2020
accepted:
04
01
2021
pubmed:
29
1
2021
medline:
28
5
2021
entrez:
28
1
2021
Statut:
ppublish
Résumé
The arterial intima is continuously under pulsatile wall shear stresses (WSS) imposed by the circulating blood. The knowledge of the contribution of smooth muscle cells (SMC) to the response of endothelial cell (EC) to WSS is still incomplete. We developed a co-culture model of EC on top of SMC that mimics the inner in vivo structure of the arterial intima of large arteries. The co-cultured model, as well as a monolayer model of EC, were developed in custom-designed wells that allowed for mechanobiology experiments. Both the monolayer and co-culture models were exposed to steady flow induced WSS of up to 24 dyne/cm
Identifiants
pubmed: 33508722
pii: S0021-9290(21)00016-6
doi: 10.1016/j.jbiomech.2021.110236
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
110236Informations de copyright
Copyright © 2021 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.