Expansion sequencing: Spatially precise in situ transcriptomics in intact biological systems.
Journal
Science (New York, N.Y.)
ISSN: 1095-9203
Titre abrégé: Science
Pays: United States
ID NLM: 0404511
Informations de publication
Date de publication:
29 01 2021
29 01 2021
Historique:
received:
08
03
2019
revised:
13
05
2020
accepted:
20
11
2020
entrez:
29
1
2021
pubmed:
30
1
2021
medline:
27
2
2021
Statut:
ppublish
Résumé
Methods for highly multiplexed RNA imaging are limited in spatial resolution and thus in their ability to localize transcripts to nanoscale and subcellular compartments. We adapt expansion microscopy, which physically expands biological specimens, for long-read untargeted and targeted in situ RNA sequencing. We applied untargeted expansion sequencing (ExSeq) to the mouse brain, which yielded the readout of thousands of genes, including splice variants. Targeted ExSeq yielded nanoscale-resolution maps of RNAs throughout dendrites and spines in the neurons of the mouse hippocampus, revealing patterns across multiple cell types, layer-specific cell types across the mouse visual cortex, and the organization and position-dependent states of tumor and immune cells in a human metastatic breast cancer biopsy. Thus, ExSeq enables highly multiplexed mapping of RNAs from nanoscale to system scale.
Identifiants
pubmed: 33509999
pii: 371/6528/eaax2656
doi: 10.1126/science.aax2656
pmc: PMC7900882
mid: NIHMS1670746
pii:
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NHGRI NIH HHS
ID : P50 HG005550
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH114031
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS102727
Pays : United States
Organisme : NCI NIH HHS
ID : HHSN261201500003C
Pays : United States
Organisme : NIBIB NIH HHS
ID : T32 EB001680
Pays : United States
Organisme : NINDS NIH HHS
ID : UF1 NS107697
Pays : United States
Organisme : NIBIB NIH HHS
ID : R01 EB024261
Pays : United States
Organisme : NCI NIH HHS
ID : HHSN261201500003I
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH110932
Pays : United States
Organisme : NIDA NIH HHS
ID : R01 DA045549
Pays : United States
Organisme : NIMH NIH HHS
ID : U19 MH114821
Pays : United States
Organisme : NHGRI NIH HHS
ID : RM1 HG008525
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH103910
Pays : United States
Organisme : NCI NIH HHS
ID : T32 CA009172
Pays : United States
Investigateurs
H R Ali
(HR)
M Al Sa'd
(M)
S Alon
(S)
S Aparicio
(S)
G Battistoni
(G)
S Balasubramanian
(S)
R Becker
(R)
B Bodenmiller
(B)
E S Boyden
(ES)
D Bressan
(D)
A Bruna
(A)
Marcel Burger
(M)
C Caldas
(C)
M Callari
(M)
I G Cannell
(IG)
H Casbolt
(H)
N Chornay
(N)
Y Cui
(Y)
A Dariush
(A)
K Dinh
(K)
A Emenari
(A)
Y Eyal-Lubling
(Y)
J Fan
(J)
A Fatemi
(A)
E Fisher
(E)
E A González-Solares
(EA)
C González-Fernández
(C)
D Goodwin
(D)
W Greenwood
(W)
F Grimaldi
(F)
G J Hannon
(GJ)
O Harris
(O)
S Harris
(S)
C Jauset
(C)
J A Joyce
(JA)
E D Karagiannis
(ED)
T Kovačević
(T)
L Kuett
(L)
R Kunes
(R)
A Küpcü Yoldaş
(A)
D Lai
(D)
E Laks
(E)
H Lee
(H)
M Lee
(M)
G Lerda
(G)
Y Li
(Y)
A McPherson
(A)
N Millar
(N)
C M Mulvey
(CM)
F Nugent
(F)
C H O'Flanagan
(CH)
M Paez-Ribes
(M)
I Pearsall
(I)
F Qosaj
(F)
A J Roth
(AJ)
O M Rueda
(OM)
T Ruiz
(T)
K Sawicka
(K)
L A Sepúlveda
(LA)
S P Shah
(SP)
A Shea
(A)
A Sinha
(A)
A Smith
(A)
S Tavaré
(S)
S Tietscher
(S)
I Vázquez-García
(I)
S L Vogl
(SL)
N A Walton
(NA)
A T Wassie
(AT)
S S Watson
(SS)
J Weselak
(J)
S A Wild
(SA)
E Williams
(E)
J Windhager
(J)
T Whitmarsh
(T)
C Xia
(C)
P Zheng
(P)
X Zhuang
(X)
Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.
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