A novel QCM immunosensor development based on gold nanoparticles functionalized sulfur-doped graphene quantum dot and h-ZnS-CdS NC for Interleukin-6 detection.

Interleukin 6 (IL-6) acts as both a proinflammatory and anti-inflammatory cytokine and is generally utilized as an important diagnostic biomarker for sepsis. In addition, the high levels of IL-6 measured in plasma have been associated with pathological inflammation. A novel quartz crystal microbalance (QCM) immunoassay method was presented for high sensitivity and selectivity detection of interleukin-6 (IL-6) based on gold nanoparticles functionalized sulfur-doped graphene quantum dot (AuNPs/S-GQD) and hollow ZnS-CdS nanocage (h-ZnS-CdS NC). Firstly, AuNPs/S-GQD nanocomposite was synthesized in the presence of tetrachloroauric acid and then conjugated onto anti-IL-6 antibodies by amino-gold affinity. The sandwich-type QCM immunoassay probe was prepared by immune-reaction between AuNPs/S-GQD/QCM immobilized with anti-IL-6 capture antibodies and h-ZnS-CdS NC including detection anti-IL-6 antibodies in the presence of target IL-6. The prepared QCM immunoassay probe was characterized by transmission electron microscopy (TEM), scanning electron microscope (SEM), x-ray diffraction (XRD) method, x-ray photoelectron spectroscopy (XPS), Raman spectroscopy, UV-vis spectroscopy, Fourier transform infrared spectroscopy (FTIR), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The QCM immunosensor showed a linearity range (0.01-2.0 pg mL

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Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. The paper is original and is not published previously in whole or a part and not being considered elsewhere.