Translocation of DNA through Ultrathin Nanoslits.
2D nanoslits
DNA translocation
biopolymers
graphene
nanofluidics
Journal
Advanced materials (Deerfield Beach, Fla.)
ISSN: 1521-4095
Titre abrégé: Adv Mater
Pays: Germany
ID NLM: 9885358
Informations de publication
Date de publication:
Mar 2021
Mar 2021
Historique:
revised:
03
12
2020
received:
11
11
2020
pubmed:
2
2
2021
medline:
14
10
2021
entrez:
1
2
2021
Statut:
ppublish
Résumé
2D nanoslit devices, where two crystals with atomically flat surfaces are separated by only a few nanometers, have attracted considerable attention because their tunable control over the confinement allows for the discovery of unusual transport behavior of gas, water, and ions. Here, the passage of double-stranded DNA molecules is studied through nanoslits fabricated from exfoliated 2D materials, such as graphene or hexagonal boron nitride, and the DNA polymer behavior is examined in this tight confinement. Two types of events are observed in the ionic current: long current blockades that signal DNA translocation and short spikes where DNA enters the slits but withdraws. DNA translocation events exhibit three distinct phases in their current-blockade traces-loading, translation, and exit. Coarse-grained molecular dynamics simulation allows the different polymer configurations of these phases to be identified. DNA molecules, including folds and knots in their polymer structure, are observed to slide through the slits with near-uniform velocity without noticeable frictional interactions of DNA with the confining graphene surfaces. It is anticipated that this new class of 2D-nanoslit devices will provide unique ways to study polymer physics and enable lab-on-a-chip biotechnology.
Identifiants
pubmed: 33522015
doi: 10.1002/adma.202007682
pmc: PMC8011289
mid: NIHMS1673653
doi:
Substances chimiques
Graphite
7782-42-5
DNA
9007-49-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2007682Subventions
Organisme : Netherlands Organization for Scientific Research
Organisme : Frontiers of Nanoscience and Basyc programs
Organisme : European Research Council
ID : 669598
Pays : International
Organisme : ERC
ID : 669598
Organisme : NIH HHS
ID : P41-GM104601
Pays : United States
Organisme : European Union's H2020 Framework Programme/ERC
ID : 852674-AngstroCAP
Organisme : NIGMS NIH HHS
ID : P41 GM104601
Pays : United States
Organisme : National Science Foundation
ID : DMR-1827346
Informations de copyright
© 2021 The Authors. Advanced Materials published by Wiley-VCH GmbH.
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