Translational Regulation of Clock Genes BMAL1 and REV-ERBα by Polyamines.
5' Untranslated Regions
/ drug effects
ARNTL Transcription Factors
/ chemistry
Animals
Gene Expression Regulation
/ drug effects
Mice
Molecular Conformation
NIH 3T3 Cells
Nuclear Receptor Subfamily 1, Group D, Member 1
/ chemistry
Polyamines
/ pharmacology
Protein Biosynthesis
/ drug effects
RNA, Messenger
/ chemistry
BMAL1
REV-ERBα
circadian clock
polyamine modulon
translation
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
28 Jan 2021
28 Jan 2021
Historique:
received:
13
01
2021
accepted:
26
01
2021
entrez:
2
2
2021
pubmed:
3
2
2021
medline:
4
9
2021
Statut:
epublish
Résumé
Polyamines stimulate the synthesis of specific proteins at the level of translation, and the genes encoding these proteins are termed as the "polyamine modulon". The circadian clock generates daily rhythms in mammalian physiology and behavior. We investigated the role of polyamines in the circadian rhythm using control and polyamine-reduced NIH3T3 cells. The intracellular polyamines exhibited a rhythm with a period of about 24 h. In the polyamine-reduced NIH3T3 cells, the circadian period of circadian clock genes was lengthened and the synthesis of BMAL1 and REV-ERBα was significantly reduced at the translation level. Thus, the mechanism of polyamine stimulation of these protein syntheses was analyzed using NIH3T3 cells transiently transfected with genes encoding enhanced green fluorescent protein (EGFP) fusion mRNA with normal or mutated 5'-untranslated region (5'-UTR) of
Identifiants
pubmed: 33525630
pii: ijms22031307
doi: 10.3390/ijms22031307
pmc: PMC7865260
pii:
doi:
Substances chimiques
5' Untranslated Regions
0
ARNTL Transcription Factors
0
Bmal1 protein, mouse
0
Nr1d1 protein, mouse
0
Nuclear Receptor Subfamily 1, Group D, Member 1
0
Polyamines
0
RNA, Messenger
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Japan Society for the Promotion of Science
ID : 19K16357 and 16K08244
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