A glucose meter interface for point-of-care gene circuit-based diagnostics.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
01 02 2021
01 02 2021
Historique:
received:
26
03
2020
accepted:
01
12
2020
entrez:
2
2
2021
pubmed:
3
2
2021
medline:
20
2
2021
Statut:
epublish
Résumé
Recent advances in cell-free synthetic biology have given rise to gene circuit-based sensors with the potential to provide decentralized and low-cost molecular diagnostics. However, it remains a challenge to deliver this sensing capacity into the hands of users in a practical manner. Here, we leverage the glucose meter, one of the most widely available point-of-care sensing devices, to serve as a universal reader for these decentralized diagnostics. We describe a molecular translator that can convert the activation of conventional gene circuit-based sensors into a glucose output that can be read by off-the-shelf glucose meters. We show the development of new glucogenic reporter systems, multiplexed reporter outputs and detection of nucleic acid targets down to the low attomolar range. Using this glucose-meter interface, we demonstrate the detection of a small-molecule analyte; sample-to-result diagnostics for typhoid, paratyphoid A/B; and show the potential for pandemic response with nucleic acid sensors for SARS-CoV-2.
Identifiants
pubmed: 33526784
doi: 10.1038/s41467-020-20639-6
pii: 10.1038/s41467-020-20639-6
pmc: PMC7851131
doi:
Substances chimiques
Nucleic Acids
0
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
724Subventions
Organisme : CIHR
ID : 201610FDN-375469
Pays : Canada
Organisme : CIHR
ID : 950-231075
Pays : Canada
Organisme : NCRR NIH HHS
ID : P41 RR005351
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI136571
Pays : United States
Organisme : NIAID NIH HHS
ID : U01 AI148319
Pays : United States
Organisme : NIGMS NIH HHS
ID : DP2 GM126892
Pays : United States
Références
Nature. 1991 Mar 7;350(6313):91-2
pubmed: 1706072
FEMS Microbiol Rev. 2005 Apr;29(2):263-79
pubmed: 15808744
Synth Syst Biotechnol. 2018 Apr 17;3(2):105-112
pubmed: 29900423
FEMS Microbiol Lett. 2003 Dec 5;229(1):97-101
pubmed: 14659548
Cell. 2016 Sep 22;167(1):248-259.e12
pubmed: 27662092
Chembiochem. 2007 Dec 17;8(18):2295-301
pubmed: 17990264
Nucleic Acids Res. 2012 Apr;40(8):3763-74
pubmed: 22180537
Nat Commun. 2018 Jul 12;9(1):2686
pubmed: 30002445
Nat Rev Cancer. 2019 Apr;19(4):187-195
pubmed: 30837696
Sci Adv. 2018 Aug 01;4(8):eaat5107
pubmed: 30083609
Chem Rev. 2008 Feb;108(2):814-25
pubmed: 18154363
Biotechnol Biofuels. 2018 Jun 30;11:185
pubmed: 29988523
N Engl J Med. 2020 Feb 20;382(8):727-733
pubmed: 31978945
Metab Eng. 2012 May;14(3):189-95
pubmed: 22314049
Chem Commun (Camb). 2013 Jan 21;49(6):585-7
pubmed: 23208450
Nat Chem. 2011 Jul 24;3(9):697-703
pubmed: 21860458
mBio. 2018 Feb 20;9(1):
pubmed: 29463654
Cell. 2016 May 19;165(5):1255-1266
pubmed: 27160350
ACS Synth Biol. 2017 Dec 15;6(12):2293-2301
pubmed: 28981256
Proc Natl Acad Sci U S A. 2012 Jun 26;109(26):E1705-14
pubmed: 22679283
Foodborne Pathog Dis. 2015 Sep;12(9):778-86
pubmed: 26270463
Int J Med Microbiol. 2005 Jun;295(2):117-20
pubmed: 15969472
Biotechniques. 2014 Apr 01;56(4):186-93
pubmed: 24724844
Biochem Eng J. 2018 Oct 15;138:91-97
pubmed: 30740032
Angew Chem Int Ed Engl. 2018 Jul 26;57(31):9702-9706
pubmed: 29893502
Nat Chem. 2020 Jan;12(1):48-55
pubmed: 31767994
ACS Synth Biol. 2012 Jan 20;1(1):29-41
pubmed: 23651008
J Clin Microbiol. 2008 May;46(5):1861-6
pubmed: 18367574
N Engl J Med. 2020 Mar 5;382(10):929-936
pubmed: 32004427
Sci Adv. 2018 Aug 01;4(8):eaat5105
pubmed: 30083608
Cell. 2014 Nov 6;159(4):940-54
pubmed: 25417167
ACS Synth Biol. 2014 Jun 20;3(6):387-97
pubmed: 24303785
Toxicol Appl Pharmacol. 2018 Apr 15;345:19-25
pubmed: 29499249
Nat Commun. 2018 Aug 21;9(1):3347
pubmed: 30131493
ACS Synth Biol. 2020 Mar 20;9(3):671-677
pubmed: 32078765
Nat Chem Biol. 2019 Jul;15(7):690-698
pubmed: 31110305
Nat Biomed Eng. 2019 Jun;3(6):427-437
pubmed: 31097816
ACS Synth Biol. 2019 Apr 19;8(4):902-905
pubmed: 30790518
ACS Synth Biol. 2019 Feb 15;8(2):455-462
pubmed: 30632751
Trends Biotechnol. 2020 Jul;38(7):766-778
pubmed: 31983463
Nat Commun. 2019 Nov 27;10(1):5404
pubmed: 31776339
Sci Rep. 2015 Jun 08;5:11039
pubmed: 26050646
J Vis Exp. 2013 Sep 16;(79):e50762
pubmed: 24084388
Anal Chem. 2012 Feb 21;84(4):1975-80
pubmed: 22235863
Cell. 2014 Nov 6;159(4):925-39
pubmed: 25417166
ACS Synth Biol. 2017 Dec 15;6(12):2198-2208
pubmed: 28795570
Nat Rev Microbiol. 2010 Jul;8(7):511-22
pubmed: 20514043
Mol Biosyst. 2014 May;10(5):970-1003
pubmed: 24643211
Lancet Infect Dis. 2019 Apr;19(4):369-381
pubmed: 30792131
Science. 2019 Jan 4;363(6422):
pubmed: 30606819
BMC Infect Dis. 2010 Mar 05;10:45
pubmed: 20205702
Synth Biol (Oxf). 2018;3(1):ysy018
pubmed: 30370338
Anal Chem. 2017 Mar 21;89(6):3395-3401
pubmed: 28238262
J R Soc Interface. 2017 Apr;14(129):
pubmed: 28446704
Nature. 2017 Aug 3;548(7665):117-121
pubmed: 28746304
Chem Commun (Camb). 2020 Feb 25;56(16):2483-2486
pubmed: 32002523
ACS Synth Biol. 2016 Apr 15;5(4):344-55
pubmed: 26818434
Nat Methods. 2009 May;6(5):343-5
pubmed: 19363495
Micromachines (Basel). 2020 Mar 14;11(3):
pubmed: 32183357