Plastidial and cytosolic thiol reductases participate in the control of stomatal functioning.
Abscisic Acid
/ metabolism
Arabidopsis
/ enzymology
Cytosol
/ metabolism
Gene Expression Regulation, Plant
Gene Ontology
Hydrogen Peroxide
/ metabolism
Models, Biological
Mutation
/ genetics
Oxidoreductases
/ metabolism
Phenotype
Plant Stomata
/ cytology
Plastids
/ metabolism
Transcriptome
/ genetics
Arabidopsis thaliana
glutaredoxin
guard cell
peroxiredoxin
proteomics
signalling
stomata
thioredoxin
Journal
Plant, cell & environment
ISSN: 1365-3040
Titre abrégé: Plant Cell Environ
Pays: United States
ID NLM: 9309004
Informations de publication
Date de publication:
05 2021
05 2021
Historique:
revised:
08
01
2021
received:
02
07
2020
accepted:
11
01
2021
pubmed:
5
2
2021
medline:
2
9
2021
entrez:
4
2
2021
Statut:
ppublish
Résumé
Stomatal movements via the control of gas exchanges determine plant growth in relation to environmental stimuli through a complex signalling network involving reactive oxygen species that lead to post-translational modifications of Cys and Met residues, and alter protein activity and/or conformation. Thiol-reductases (TRs), which include thioredoxins, glutaredoxins (GRXs) and peroxiredoxins (PRXs), participate in signalling pathways through the control of Cys redox status in client proteins. Their involvement in stomatal functioning remains poorly characterized. By performing a mass spectrometry-based proteomic analysis, we show that numerous thiol reductases, like PRXs, are highly abundant in guard cells. When investigating various Arabidopsis mutants impaired in the expression of TR genes, no change in stomatal density and index was noticed. In optimal growth conditions, a line deficient in cytosolic NADPH-thioredoxin reductases displayed higher stomatal conductance and lower leaf temperature evaluated by thermal infrared imaging. In contrast, lines deficient in plastidial 2-CysPRXs or type-II GRXs exhibited compared to WT reduced conductance and warmer leaves in optimal conditions, and enhanced stomatal closure in epidermal peels treated with abscisic acid or hydrogen peroxide. Altogether, these data strongly support the contribution of thiol redox switches within the signalling network regulating guard cell movements and stomatal functioning.
Substances chimiques
Abscisic Acid
72S9A8J5GW
Hydrogen Peroxide
BBX060AN9V
Oxidoreductases
EC 1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1417-1435Informations de copyright
© 2021 John Wiley & Sons Ltd.
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