Mapping the origin and fate of myeloid cells in distinct compartments of the eye by single-cell profiling.
Animals
Choroid
/ blood supply
Computational Biology
Computer Simulation
Eye
/ cytology
Female
Homeostasis
/ immunology
Humans
Immunity, Innate
/ immunology
Macrophages
/ immunology
Macular Degeneration
/ pathology
Male
Mice
Mice, Inbred C57BL
Mice, Transgenic
Microglia
/ physiology
Myeloid Cells
/ immunology
Neovascularization, Physiologic
/ physiology
Sequence Analysis, RNA
Single-Cell Analysis
Transcription, Genetic
/ genetics
cornea
macrophages
microglia
retina
single-cell RNA-seq
Journal
The EMBO journal
ISSN: 1460-2075
Titre abrégé: EMBO J
Pays: England
ID NLM: 8208664
Informations de publication
Date de publication:
15 03 2021
15 03 2021
Historique:
revised:
07
12
2020
received:
27
03
2020
accepted:
18
12
2020
pubmed:
9
2
2021
medline:
21
10
2021
entrez:
8
2
2021
Statut:
ppublish
Résumé
Similar to the brain, the eye is considered an immune-privileged organ where tissue-resident macrophages provide the major immune cell constituents. However, little is known about spatially restricted macrophage subsets within different eye compartments with regard to their origin, function, and fate during health and disease. Here, we combined single-cell analysis, fate mapping, parabiosis, and computational modeling to comprehensively examine myeloid subsets in distinct parts of the eye during homeostasis. This approach allowed us to identify myeloid subsets displaying diverse transcriptional states. During choroidal neovascularization, a typical hallmark of neovascular age-related macular degeneration (AMD), we recognized disease-specific macrophage subpopulations with distinct molecular signatures. Our results highlight the heterogeneity of myeloid subsets and their dynamics in the eye that provide new insights into the innate immune system in this organ which may offer new therapeutic targets for ophthalmological diseases.
Identifiants
pubmed: 33555074
doi: 10.15252/embj.2020105123
pmc: PMC7957431
doi:
Banques de données
GEO
['GSE160845', 'GSE160797']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e105123Subventions
Organisme : Medical Research Council
ID : MC_PC_16031
Pays : United Kingdom
Commentaires et corrections
Type : CommentIn
Informations de copyright
© 2021 The Authors. Published under the terms of the CC BY NC ND 4.0 license.
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