Recombinant production of poly-(3-hydroxybutyrate) by Bacillus megaterium utilizing millet bran and rapeseed meal hydrolysates.

3-Hydroxybutyric Acid Bacillus megaterium Brassica napus Hydroxybutyrates Kinetics Millets Polyesters
Bacillus megaterium Kinetic modeling Metabolic engineering Millet bran hydrolysate Poly-3-hydroxybutyrate

Journal

Bioresource technology
ISSN: 1873-2976
Titre abrégé: Bioresour Technol
Pays: England
ID NLM: 9889523

Informations de publication

Date de publication:
Apr 2021
Historique:
received: 20 12 2020
revised: 25 01 2021
accepted: 27 01 2021
pubmed: 9 2 2021
medline: 20 2 2021
entrez: 8 2 2021
Statut: ppublish

Résumé

Fermentative poly-3-hydroxybutyrate (PHB) production is mainly limited by the cost of raw material. In this present study, low-cost feedstock viz., millet bran residue (MBRH) and rapeseed meal hydrolysates were successfully utilized for PHB production. Metabolic engineering of Bacillus megaterium by co-expression of both precursor (phbRBC) and NADPH cofactor regeneration (zwf) genes resulted in 2.67-fold enhancement in PHB accumulation compared to wild strain. Modified logistic model characterized B.megaterium growth and PHB production effectively. The kinetic analysis proved that maximum cell concentration (15.01 g.L

Identifiants

DOI: 10.1016/j.biortech.2021.124800 PMID: 33556706
pubmed: 33556706
pii: S0960-8524(21)00139-5
doi: 10.1016/j.biortech.2021.124800
pii:
doi:

Substances chimiques

Hydroxybutyrates 0
Polyesters 0
3-Hydroxybutyric Acid TZP1275679

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

124800

Informations de copyright

Copyright © 2021 Elsevier Ltd. All rights reserved.

Auteurs

Subbi Rami Reddy Tadi (S)

BioPAT Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India.

Som Dutt Ravindran (S)

Department of Biotechnology, Manipal Institute of Technology, Manipal 576104, Karnataka, India.

Rengesh Balakrishnan (R)

Department of Pharmaceutical Technology, Mahendra Engineering College, Namakkal District 637503, Tamil Nadu, India.

Senthilkumar Sivaprakasam (S)

BioPAT Laboratory, Department of Biosciences and Bioengineering, Indian Institute of Technology Guwahati, Guwahati 781039, Assam, India. Electronic address: senthilkumar@iitg.ac.in.

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Classifications MeSH

questionsmedicales.fr › Lipides › Acides gras › Acides gras volatils › Butyrates › Hydroxy-butyrates › Acide 3-hydroxy-butyrique › Recombinant production of...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets sporulés à Gram positif › Bacillaceae › Bacillus › Bacillus megaterium › Recombinant production of...
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Brassicaceae › Brassica › Brassica napus › Recombinant production of...
questionsmedicales.fr › Lipides › Acides gras › Acides gras volatils › Butyrates › Hydroxy-butyrates › Recombinant production of...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Cinétique › Recombinant production of...
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Poaceae › Millets › Recombinant production of...
questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomédicaux et dentaires › Polymères › Polyesters › Recombinant production of...