Chromosomal assembly of the nuclear genome of the endosymbiont-bearing trypanosomatid Angomonas deanei.

Angomonas deanei Carvalho (ATCC® PRA-265™) Oxford Nanopore genome assembly

Journal

G3 (Bethesda, Md.)
ISSN: 2160-1836
Titre abrégé: G3 (Bethesda)
Pays: England
ID NLM: 101566598

Informations de publication

Date de publication:
18 01 2021
Historique:
received: 19 08 2020
accepted: 17 11 2020
entrez: 9 2 2021
pubmed: 10 2 2021
medline: 7 7 2021
Statut: ppublish

Résumé

Angomonas deanei is an endosymbiont-bearing trypanosomatid with several highly fragmented genome assemblies and unknown chromosome number. We present an assembly of the A. deanei nuclear genome based on Oxford Nanopore sequence that resolves into 29 complete or close-to-complete chromosomes. The assembly has several previously unknown special features; it has a supernumerary chromosome, a chromosome with a 340-kb inversion, and there is a translocation between two chromosomes. We also present an updated annotation of the chromosomal genome with 10,365 protein-coding genes, 59 transfer RNAs, 26 ribosomal RNAs, and 62 noncoding RNAs.

Identifiants

pubmed: 33561222
pii: 6007473
doi: 10.1093/g3journal/jkaa018
pmc: PMC8022732
pii:
doi:

Banques de données

figshare
['10.25387/g3.13252664.']

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 200807/Z/16/Z
Pays : United Kingdom

Informations de copyright

© The Author(s) 2020. Published by Oxford University Press on behalf of Genetics Society of America.

Références

Mem Inst Oswaldo Cruz. 2016 Nov;111(11):686-691
pubmed: 27706380
Protist. 2011 Jul;162(3):503-24
pubmed: 21420905
mBio. 2016 Mar 15;7(2):e01985
pubmed: 26980834
Nat Rev Microbiol. 2007 Jan;5(1):70-5
pubmed: 17160000
Protist. 2014 Dec;165(6):825-38
pubmed: 25460233
Eukaryot Cell. 2002 Aug;1(4):495-502
pubmed: 12455998
Emerg Infect Dis. 2019 Nov;25(11):2088-2092
pubmed: 31625841
BMC Evol Biol. 2016 Nov 11;16(1):247
pubmed: 27835948
Mem Inst Oswaldo Cruz. 1998 Jul-Aug;93(4):531-7
pubmed: 9711346
PLoS One. 2016 Oct 5;11(10):e0163962
pubmed: 27706213
Protist. 2017 Apr;168(2):253-269
pubmed: 28371652
FEMS Microbiol Lett. 2007 Oct;275(2):229-36
pubmed: 17714482
BMC Evol Biol. 2013 Sep 09;13:190
pubmed: 24015778
PLoS One. 2014 Nov 19;9(11):e112963
pubmed: 25409509
PLoS One. 2010 Aug 26;5(8):e12415
pubmed: 20865129
Bioinformatics. 2009 Aug 15;25(16):2078-9
pubmed: 19505943
Brief Bioinform. 2013 Mar;14(2):178-92
pubmed: 22517427
J Eukaryot Microbiol. 2016 Nov;63(6):794-803
pubmed: 27194398
Vector Borne Zoonotic Dis. 2011 Jul;11(7):835-41
pubmed: 21142963
PLoS One. 2013 Nov 19;8(11):e79786
pubmed: 24260300
Genome Res. 2011 Dec;21(12):2129-42
pubmed: 22038252
J Parasitol. 1960 Oct;46:529-39
pubmed: 13783227
Nucleic Acids Res. 2016 Jul 8;44(W1):W29-34
pubmed: 27105845
Bioinformatics. 2018 Mar 1;34(5):867-868
pubmed: 29096012
Genome Res. 2011 Dec;21(12):2143-56
pubmed: 22038251
Genome Res. 2017 May;27(5):722-736
pubmed: 28298431
Bioinformatics. 2011 Nov 1;27(21):2987-93
pubmed: 21903627
PLoS One. 2013;8(4):e60209
pubmed: 23560078
J Clin Microbiol. 2012 Aug;50(8):2774-8
pubmed: 22622439
Infect Genet Evol. 2010 Oct;10(7):1145-50
pubmed: 20633704
Curr Genomics. 2018 Feb;19(2):87-97
pubmed: 29491737
Front Plant Sci. 2019 Apr 16;10:475
pubmed: 31057581
Bioinformatics. 2018 Sep 15;34(18):3094-3100
pubmed: 29750242
PLoS Pathog. 2015 Aug 28;11(8):e1005127
pubmed: 26317207
Mol Ecol. 2017 Jun;26(11):2864-2879
pubmed: 28220980

Auteurs

John W Davey (JW)

Department of Biology, University of York, York YO10 5DD, UK.

Carolina M C Catta-Preta (CMC)

Department of Biology, University of York, York YO10 5DD, UK.
York Biomedical Research Institute, University of York, York YO10 5DD, UK.
Medicinal Chemistry Center (CQMED)/Structural Genomics Consortium, Universidade Estadual de Campinas, Campinas, São Paulo 13083-886, Brazil.

Sally James (S)

Department of Biology, University of York, York YO10 5DD, UK.

Sarah Forrester (S)

Department of Biology, University of York, York YO10 5DD, UK.

Maria Cristina M Motta (MCM)

Laboratório de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Departamento de Biologia Celular e Parasitologia, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Instituto Nacional de Ciência e Tecnologia em Biologia Estrutural e Bioimagens, Rio de Janeiro, RJ, Brazil.

Peter D Ashton (PD)

Department of Biology, University of York, York YO10 5DD, UK.

Jeremy C Mottram (JC)

Department of Biology, University of York, York YO10 5DD, UK.
York Biomedical Research Institute, University of York, York YO10 5DD, UK.

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