A Genetically Encoded Bioluminescence Intracellular Nanosensor for Androgen Receptor Activation Monitoring in 3D Cell Models.
3D cell model
androgen receptor
bioluminescence
complementation assay
luciferase
Journal
Sensors (Basel, Switzerland)
ISSN: 1424-8220
Titre abrégé: Sensors (Basel)
Pays: Switzerland
ID NLM: 101204366
Informations de publication
Date de publication:
29 Jan 2021
29 Jan 2021
Historique:
received:
05
01
2021
revised:
25
01
2021
accepted:
26
01
2021
entrez:
12
2
2021
pubmed:
13
2
2021
medline:
6
3
2021
Statut:
epublish
Résumé
In recent years, there has been an increasing demand for predictive and sensitive in vitro tools for drug discovery. Split complementation assays have the potential to enlarge the arsenal of in vitro tools for compound screening, with most of them relying on well-established reporter gene assays. In particular, ligand-induced complementation of split luciferases is emerging as a suitable approach for monitoring protein-protein interactions. We hereby report an intracellular nanosensor for the screening of compounds with androgenic activity based on a split NanoLuc reporter. We also confirm the suitability of using 3D spheroids of Human Embryonic Kidney (HEK-293) cells for upgrading the 2D cell-based assay. A limit of detection of 4 pM and a half maximal effective concentration (EC
Identifiants
pubmed: 33572727
pii: s21030893
doi: 10.3390/s21030893
pmc: PMC7865915
pii:
doi:
Substances chimiques
Androgens
0
Receptors, Androgen
0
Luciferases
EC 1.13.12.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Ministero dell'Istruzione, dell'Università e della Ricerca
ID : 2015FFY97L
Organisme : Ministero dell'Istruzione, dell'Università e della Ricerca
ID : 2017Y2PAB8
Organisme : North Atlantic Treaty Organization
ID : 985042
Organisme : Partnership for Research and Innovation in the Mediterranean Area
ID : FEDKITO
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